Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa

Francesco Luzzaro, Andrea Endimiani, Jean Denis Docquier, Claudia Mugnaioli, Mattia Bonsignori, Gianfranco Amicosante, Gian Maria Rossolini, Antonio Toniolo

Research output: Contribution to journalArticle

Abstract

The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes.

Original languageEnglish
Pages (from-to)131-135
Number of pages5
JournalDiagnostic Microbiology and Infectious Disease
Volume48
Issue number2
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Pseudomonas aeruginosa
Disk Diffusion Antimicrobial Tests
Genes
Inosine Monophosphate
Carbapenems
Ceftazidime
Imipenem
DNA Probes
Microbial Sensitivity Tests
Chelating Agents
DNA Sequence Analysis
Sweden
Edetic Acid
Polymerase Chain Reaction
Enzymes
VIM-2 determinant
1,10-phenanthroline

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Luzzaro, F., Endimiani, A., Docquier, J. D., Mugnaioli, C., Bonsignori, M., Amicosante, G., ... Toniolo, A. (2004). Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa. Diagnostic Microbiology and Infectious Disease, 48(2), 131-135. https://doi.org/10.1016/j.diagmicrobio.2003.09.005

Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa. / Luzzaro, Francesco; Endimiani, Andrea; Docquier, Jean Denis; Mugnaioli, Claudia; Bonsignori, Mattia; Amicosante, Gianfranco; Rossolini, Gian Maria; Toniolo, Antonio.

In: Diagnostic Microbiology and Infectious Disease, Vol. 48, No. 2, 02.2004, p. 131-135.

Research output: Contribution to journalArticle

Luzzaro, F, Endimiani, A, Docquier, JD, Mugnaioli, C, Bonsignori, M, Amicosante, G, Rossolini, GM & Toniolo, A 2004, 'Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa', Diagnostic Microbiology and Infectious Disease, vol. 48, no. 2, pp. 131-135. https://doi.org/10.1016/j.diagmicrobio.2003.09.005
Luzzaro, Francesco ; Endimiani, Andrea ; Docquier, Jean Denis ; Mugnaioli, Claudia ; Bonsignori, Mattia ; Amicosante, Gianfranco ; Rossolini, Gian Maria ; Toniolo, Antonio. / Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa. In: Diagnostic Microbiology and Infectious Disease. 2004 ; Vol. 48, No. 2. pp. 131-135.
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