Abstract
The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes.
| Original language | English |
|---|---|
| Pages (from-to) | 131-135 |
| Number of pages | 5 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Volume | 48 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2004 |
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ASJC Scopus subject areas
- Infectious Diseases
- Immunology and Allergy
- Virology
- Parasitology
- Microbiology
- Immunology
- Applied Microbiology and Biotechnology
Cite this
Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa. / Luzzaro, Francesco; Endimiani, Andrea; Docquier, Jean Denis; Mugnaioli, Claudia; Bonsignori, Mattia; Amicosante, Gianfranco; Rossolini, Gian Maria; Toniolo, Antonio.
In: Diagnostic Microbiology and Infectious Disease, Vol. 48, No. 2, 02.2004, p. 131-135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Prevalence and characterization of metallo-β-lactamases in clinical isolates of Pseudomonas aeruginosa
AU - Luzzaro, Francesco
AU - Endimiani, Andrea
AU - Docquier, Jean Denis
AU - Mugnaioli, Claudia
AU - Bonsignori, Mattia
AU - Amicosante, Gianfranco
AU - Rossolini, Gian Maria
AU - Toniolo, Antonio
PY - 2004/2
Y1 - 2004/2
N2 - The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes.
AB - The prevalence and the type(s) of metallo-β-lactamases (MBLs) produced by isolates of Pseudomonas aeruginosa were investigated. During 2001, 506 nonduplicate isolates were obtained from hospitalized patients. Eighty-two strains were selected because of resistance to carbapenems and/or ceftazidime. Screening for MBL production was performed in the latter isolates by the Etest MBL strips (AB Biodisk, Solna, Sweden) and by a broth microdilution method measuring minimum inhibitory concentrations (MICs) of imipenem alone and in the presence of metal-chelating agents (EDTA and o-phenanthroline). Specific DNA probes were used to investigate the presence of genes coding for IMP- or VIM-type enzymes. Overall, four isolates of P. aeruginosa (obtained from independent patients) were found to carry a blaVIM gene. Polymerase chain reaction (PCR) experiments and DNA sequencing revealed that the VIM-2 determinant was present in three cases, whereas VIM-1 was detected in one isolate. Surveillance programs should be adopted to avoid the spread of these worrisome resistance genes.
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UR - http://www.scopus.com/inward/citedby.url?scp=1242337997&partnerID=8YFLogxK
U2 - 10.1016/j.diagmicrobio.2003.09.005
DO - 10.1016/j.diagmicrobio.2003.09.005
M3 - Article
C2 - 14972383
AN - SCOPUS:1242337997
VL - 48
SP - 131
EP - 135
JO - Diagnostic Microbiology and Infectious Disease
JF - Diagnostic Microbiology and Infectious Disease
SN - 0732-8893
IS - 2
ER -