Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners: An Italian screening study

Gianguido Cossellu, Luigi Fedele, Bouabid Badaoui, Francesca Angiero, Giampietro Farronato, Ermelinda Monti, Carlo Antonio Liverani, Chiara Gorni, Sara Botti

Research output: Contribution to journalArticle

Abstract

OBJECTIVES: This cross-sectional study aimed to evaluate the prevalence and type of oral HPV-infection in women with a cervical HPV-lesion and in the oral and genital mucosa of their male partners.

METHODS: The study group comprised 44 sexually-active women, 20-45 years with abnormal PAP smear, not more than 6 months prior to referral together with the male partners cohabiting in stable partnerships. A detailed questionnaire was administered concerning the HPV-related risk factors. Oral swabs, oral rinses, cervical swabs and urine samples were collected. HPV DNA was detected using two different polymerase chain reactions (PCRs): MY09-11 and FAP59-64. Positive samples were genotyped by Sanger sequencing and the INNO-LiPA HPV Genotyping Extra II probe assay. The association with risk factors was assessed by fitting a generalized model, using the General Linear Model function in the R-software; correlations were calculated between all data.

RESULTS: HPV was detected in 84% of Cervical Samples, in 24.3% of oral samples and in one urine sample. Only 27% of the HPV-positive results were identical with both PCR DNA assays. 8 male had oral HPV-positive samples different from women cervical samples. In one couple the urine-male sample had the same HPV present in the female-cervical sample. A significant association resulted between women/oral sex practices and men/n. of partners.

CONCLUSIONS: This study reports that women (20.4%) with a diagnosis of cervical-HPV and their male partners (30,7%) are at high risk for subclinical oral HPV infection.

Original languageEnglish
Pages (from-to)e0205574
JournalPLoS One
Volume13
Issue number10
DOIs
Publication statusPublished - Jan 1 2018

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Sexual Partners
genitalia
mouth
Screening
Polymerase chain reaction
screening
Assays
Infection
Association reactions
infection
Urine
DNA
sampling
urine
Polymerase Chain Reaction
Mouth Mucosa
Sexual Behavior
risk factors
Linear Models
polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners : An Italian screening study. / Cossellu, Gianguido; Fedele, Luigi; Badaoui, Bouabid; Angiero, Francesca; Farronato, Giampietro; Monti, Ermelinda; Liverani, Carlo Antonio; Gorni, Chiara; Botti, Sara.

In: PLoS One, Vol. 13, No. 10, 01.01.2018, p. e0205574.

Research output: Contribution to journalArticle

Cossellu, Gianguido ; Fedele, Luigi ; Badaoui, Bouabid ; Angiero, Francesca ; Farronato, Giampietro ; Monti, Ermelinda ; Liverani, Carlo Antonio ; Gorni, Chiara ; Botti, Sara. / Prevalence and concordance of oral and genital HPV in women positive for cervical HPV infection and in their sexual stable partners : An Italian screening study. In: PLoS One. 2018 ; Vol. 13, No. 10. pp. e0205574.
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AU - Angiero, Francesca

AU - Farronato, Giampietro

AU - Monti, Ermelinda

AU - Liverani, Carlo Antonio

AU - Gorni, Chiara

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N2 - OBJECTIVES: This cross-sectional study aimed to evaluate the prevalence and type of oral HPV-infection in women with a cervical HPV-lesion and in the oral and genital mucosa of their male partners.METHODS: The study group comprised 44 sexually-active women, 20-45 years with abnormal PAP smear, not more than 6 months prior to referral together with the male partners cohabiting in stable partnerships. A detailed questionnaire was administered concerning the HPV-related risk factors. Oral swabs, oral rinses, cervical swabs and urine samples were collected. HPV DNA was detected using two different polymerase chain reactions (PCRs): MY09-11 and FAP59-64. Positive samples were genotyped by Sanger sequencing and the INNO-LiPA HPV Genotyping Extra II probe assay. The association with risk factors was assessed by fitting a generalized model, using the General Linear Model function in the R-software; correlations were calculated between all data.RESULTS: HPV was detected in 84% of Cervical Samples, in 24.3% of oral samples and in one urine sample. Only 27% of the HPV-positive results were identical with both PCR DNA assays. 8 male had oral HPV-positive samples different from women cervical samples. In one couple the urine-male sample had the same HPV present in the female-cervical sample. A significant association resulted between women/oral sex practices and men/n. of partners.CONCLUSIONS: This study reports that women (20.4%) with a diagnosis of cervical-HPV and their male partners (30,7%) are at high risk for subclinical oral HPV infection.

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