Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti

Translated title of the contribution: Prevalence of HGV RNA and anti-E2 in a group of blood donors, in patients with liver diseases and in intravenous drug users

M. Mazzucco, R. Poma, F. Rizzuto, M. Pizzino, G. Belloni, R. Insolia, G. Barbarini, L. Salvaneschi

Research output: Contribution to journalArticle

Abstract

GBV-C/HGV is a newly described hepatitis virus belonging to the Flaviviridae family. The virus has a genome organisation similar to that of hepatitis C virus (HCV) with which it shares 25% identity at the nucleotide level. Probably, it is causative agent of non A-non E hepatitis and it is readily transmitted by blood transfusions, by some blood products, by parenteral exposure to blood during intravenous drug use and haemodialysis. In this study, we have investigated HGV prevalence in a group of blood donors, hepatopatic patients and intravenous drug users, according to different clinical-virological markers. Total RNA extracted from plasma samples was amplified by RT-PCR using specific primers for the 5' untranslated region (5'-UTR) and the 3-A non structural region (NS3) of the HGV genome. Using an enzyme immunoassay, we tested in the same samples the seroprevalence of antibodies to HGV envelope protein (anti-E2). The results are in accordance with known ones even if the pathological significance of HGV infection remains unelucidated. So, we propose to perform a patients follow-up and investigate biopsy by molecular techniques in order to elucidate the role of HGV in hepatic disease.

Original languageItalian
Pages (from-to)94-98
Number of pages5
JournalTrasfusione del Sangue
Volume44
Issue number2
Publication statusPublished - 1999

Fingerprint

Drug Users
Blood Donors
Liver Diseases
Flaviviridae
Genome
GB virus C
RNA
Hepatitis E
Hepatitis Viruses
5' Untranslated Regions
Seroepidemiologic Studies
Immunoenzyme Techniques
Blood Transfusion
Hepacivirus
Renal Dialysis
Nucleotides
Biomarkers
Viruses
Biopsy
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Hematology
  • Immunology and Allergy

Cite this

Mazzucco, M., Poma, R., Rizzuto, F., Pizzino, M., Belloni, G., Insolia, R., ... Salvaneschi, L. (1999). Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti. Trasfusione del Sangue, 44(2), 94-98.

Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti. / Mazzucco, M.; Poma, R.; Rizzuto, F.; Pizzino, M.; Belloni, G.; Insolia, R.; Barbarini, G.; Salvaneschi, L.

In: Trasfusione del Sangue, Vol. 44, No. 2, 1999, p. 94-98.

Research output: Contribution to journalArticle

Mazzucco, M, Poma, R, Rizzuto, F, Pizzino, M, Belloni, G, Insolia, R, Barbarini, G & Salvaneschi, L 1999, 'Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti', Trasfusione del Sangue, vol. 44, no. 2, pp. 94-98.
Mazzucco, M. ; Poma, R. ; Rizzuto, F. ; Pizzino, M. ; Belloni, G. ; Insolia, R. ; Barbarini, G. ; Salvaneschi, L. / Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti. In: Trasfusione del Sangue. 1999 ; Vol. 44, No. 2. pp. 94-98.
@article{22e4e3bee2b04f5b9096f1758f892d86,
title = "Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti",
abstract = "GBV-C/HGV is a newly described hepatitis virus belonging to the Flaviviridae family. The virus has a genome organisation similar to that of hepatitis C virus (HCV) with which it shares 25{\%} identity at the nucleotide level. Probably, it is causative agent of non A-non E hepatitis and it is readily transmitted by blood transfusions, by some blood products, by parenteral exposure to blood during intravenous drug use and haemodialysis. In this study, we have investigated HGV prevalence in a group of blood donors, hepatopatic patients and intravenous drug users, according to different clinical-virological markers. Total RNA extracted from plasma samples was amplified by RT-PCR using specific primers for the 5' untranslated region (5'-UTR) and the 3-A non structural region (NS3) of the HGV genome. Using an enzyme immunoassay, we tested in the same samples the seroprevalence of antibodies to HGV envelope protein (anti-E2). The results are in accordance with known ones even if the pathological significance of HGV infection remains unelucidated. So, we propose to perform a patients follow-up and investigate biopsy by molecular techniques in order to elucidate the role of HGV in hepatic disease.",
keywords = "Anti-E2, Blood donors, Hepatopatic patients, HGV RNA, Intravenous drug users",
author = "M. Mazzucco and R. Poma and F. Rizzuto and M. Pizzino and G. Belloni and R. Insolia and G. Barbarini and L. Salvaneschi",
year = "1999",
language = "Italian",
volume = "44",
pages = "94--98",
journal = "Trasfusione del Sangue",
issn = "0041-1787",
publisher = "SIMTI Servizi",
number = "2",

}

TY - JOUR

T1 - Prevalenza di HGV RNA ed anti-E2 in un gruppo di donatori di sangue, in pazienti epatopatici e in tossicodipendenti

AU - Mazzucco, M.

AU - Poma, R.

AU - Rizzuto, F.

AU - Pizzino, M.

AU - Belloni, G.

AU - Insolia, R.

AU - Barbarini, G.

AU - Salvaneschi, L.

PY - 1999

Y1 - 1999

N2 - GBV-C/HGV is a newly described hepatitis virus belonging to the Flaviviridae family. The virus has a genome organisation similar to that of hepatitis C virus (HCV) with which it shares 25% identity at the nucleotide level. Probably, it is causative agent of non A-non E hepatitis and it is readily transmitted by blood transfusions, by some blood products, by parenteral exposure to blood during intravenous drug use and haemodialysis. In this study, we have investigated HGV prevalence in a group of blood donors, hepatopatic patients and intravenous drug users, according to different clinical-virological markers. Total RNA extracted from plasma samples was amplified by RT-PCR using specific primers for the 5' untranslated region (5'-UTR) and the 3-A non structural region (NS3) of the HGV genome. Using an enzyme immunoassay, we tested in the same samples the seroprevalence of antibodies to HGV envelope protein (anti-E2). The results are in accordance with known ones even if the pathological significance of HGV infection remains unelucidated. So, we propose to perform a patients follow-up and investigate biopsy by molecular techniques in order to elucidate the role of HGV in hepatic disease.

AB - GBV-C/HGV is a newly described hepatitis virus belonging to the Flaviviridae family. The virus has a genome organisation similar to that of hepatitis C virus (HCV) with which it shares 25% identity at the nucleotide level. Probably, it is causative agent of non A-non E hepatitis and it is readily transmitted by blood transfusions, by some blood products, by parenteral exposure to blood during intravenous drug use and haemodialysis. In this study, we have investigated HGV prevalence in a group of blood donors, hepatopatic patients and intravenous drug users, according to different clinical-virological markers. Total RNA extracted from plasma samples was amplified by RT-PCR using specific primers for the 5' untranslated region (5'-UTR) and the 3-A non structural region (NS3) of the HGV genome. Using an enzyme immunoassay, we tested in the same samples the seroprevalence of antibodies to HGV envelope protein (anti-E2). The results are in accordance with known ones even if the pathological significance of HGV infection remains unelucidated. So, we propose to perform a patients follow-up and investigate biopsy by molecular techniques in order to elucidate the role of HGV in hepatic disease.

KW - Anti-E2

KW - Blood donors

KW - Hepatopatic patients

KW - HGV RNA

KW - Intravenous drug users

UR - http://www.scopus.com/inward/record.url?scp=0032981323&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032981323&partnerID=8YFLogxK

M3 - Articolo

AN - SCOPUS:0032981323

VL - 44

SP - 94

EP - 98

JO - Trasfusione del Sangue

JF - Trasfusione del Sangue

SN - 0041-1787

IS - 2

ER -