Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

for the SPLIT study group

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age-stratified large sample of immunocompetent children and adults. Methods: Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species-specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein-Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma-associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. Results: Overall 85% of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70%), followed by HHV6B (≈50%), HSV1, CMV, VZV (≈2%), and KSHV and HSV2 (<1%), while HHV6A was not detected. EBV prevalence was significantly higher in adults than in children, whereas it was opposite for HHV6B and VZV. No difference in HHV prevalence was observed by sex. In multivariate analysis, EBV detection was associated with age greater than or equal to 15 years (prevalence ratio [PR] = 1.8; 95% confidence interval [CI]: 1.5-2.3) and marginally with tobacco smoking (PR = 1.2; 95% CI: 1.1-1.3). Conclusion: Differing patterns of HHV infection in tonsils in a large age-stratified population were described. This study is by far the largest available and shows that EBV, HHV6B, and HHV7 are commonly detected in the tonsils in both men and women, in contrast to other HHVs.

Original languageEnglish
JournalJournal of Medical Virology
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Human Herpesvirus 6
Herpesviridae Infections
Palatine Tonsil
Human Herpesvirus 4
Human Herpesvirus 3
Herpesviridae
Human Herpesvirus 8
Human Herpesvirus 1
Cytomegalovirus
Confidence Intervals
Tonsillectomy
DNA
Multivariate Analysis
Smoking
Population

Keywords

  • epidemiology
  • human herpesvirus
  • oropharynx
  • prevalence
  • tonsil brushing

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Prevalence of human herpesviruses infections in nonmalignant tonsils : The SPLIT study. / for the SPLIT study group.

In: Journal of Medical Virology, 01.01.2018.

Research output: Contribution to journalArticle

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title = "Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study",
abstract = "Objective: To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age-stratified large sample of immunocompetent children and adults. Methods: Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species-specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein-Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma-associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. Results: Overall 85{\%} of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70{\%}), followed by HHV6B (≈50{\%}), HSV1, CMV, VZV (≈2{\%}), and KSHV and HSV2 (<1{\%}), while HHV6A was not detected. EBV prevalence was significantly higher in adults than in children, whereas it was opposite for HHV6B and VZV. No difference in HHV prevalence was observed by sex. In multivariate analysis, EBV detection was associated with age greater than or equal to 15 years (prevalence ratio [PR] = 1.8; 95{\%} confidence interval [CI]: 1.5-2.3) and marginally with tobacco smoking (PR = 1.2; 95{\%} CI: 1.1-1.3). Conclusion: Differing patterns of HHV infection in tonsils in a large age-stratified population were described. This study is by far the largest available and shows that EBV, HHV6B, and HHV7 are commonly detected in the tonsils in both men and women, in contrast to other HHVs.",
keywords = "epidemiology, human herpesvirus, oropharynx, prevalence, tonsil brushing",
author = "{for the SPLIT study group} and Aboud Kourieh and Tarik Gheit and Massimo Tommasino and V{\'e}ronique Dalstein and Clifford, {Gary M.} and {Lacau St Guily}, Jean and Christine Clavel and Silvia Franceschi and Combes, {Jean Damien}",
year = "2018",
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T1 - Prevalence of human herpesviruses infections in nonmalignant tonsils

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AU - for the SPLIT study group

AU - Kourieh, Aboud

AU - Gheit, Tarik

AU - Tommasino, Massimo

AU - Dalstein, Véronique

AU - Clifford, Gary M.

AU - Lacau St Guily, Jean

AU - Clavel, Christine

AU - Franceschi, Silvia

AU - Combes, Jean Damien

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objective: To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age-stratified large sample of immunocompetent children and adults. Methods: Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species-specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein-Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma-associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. Results: Overall 85% of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70%), followed by HHV6B (≈50%), HSV1, CMV, VZV (≈2%), and KSHV and HSV2 (<1%), while HHV6A was not detected. EBV prevalence was significantly higher in adults than in children, whereas it was opposite for HHV6B and VZV. No difference in HHV prevalence was observed by sex. In multivariate analysis, EBV detection was associated with age greater than or equal to 15 years (prevalence ratio [PR] = 1.8; 95% confidence interval [CI]: 1.5-2.3) and marginally with tobacco smoking (PR = 1.2; 95% CI: 1.1-1.3). Conclusion: Differing patterns of HHV infection in tonsils in a large age-stratified population were described. This study is by far the largest available and shows that EBV, HHV6B, and HHV7 are commonly detected in the tonsils in both men and women, in contrast to other HHVs.

AB - Objective: To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age-stratified large sample of immunocompetent children and adults. Methods: Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species-specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein-Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma-associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. Results: Overall 85% of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70%), followed by HHV6B (≈50%), HSV1, CMV, VZV (≈2%), and KSHV and HSV2 (<1%), while HHV6A was not detected. EBV prevalence was significantly higher in adults than in children, whereas it was opposite for HHV6B and VZV. No difference in HHV prevalence was observed by sex. In multivariate analysis, EBV detection was associated with age greater than or equal to 15 years (prevalence ratio [PR] = 1.8; 95% confidence interval [CI]: 1.5-2.3) and marginally with tobacco smoking (PR = 1.2; 95% CI: 1.1-1.3). Conclusion: Differing patterns of HHV infection in tonsils in a large age-stratified population were described. This study is by far the largest available and shows that EBV, HHV6B, and HHV7 are commonly detected in the tonsils in both men and women, in contrast to other HHVs.

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KW - human herpesvirus

KW - oropharynx

KW - prevalence

KW - tonsil brushing

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