Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study

Aboud Kourieh, Tarik Gheit, Massimo Tommasino, Véronique Dalstein, Gary M Clifford, Jean Lacau St Guily, Christine Clavel, Silvia Franceschi, Jean-Damien Combes, SPLIT study group

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To assess the prevalence of all known human herpesviruses (HHV) in tonsils of an age-stratified large sample of immunocompetent children and adults. METHODS: Patients undergoing tonsillectomy for benign indications were recruited in 19 French hospitals. After resection, the entire outer surfaces of right and left half tonsils were extensively brushed. A highly sensitive species-specific multiplex assay was used to detect herpes simplex virus 1 (HSV1), HSV2, Epstein-Barr virus (EBV; types 1 and 2), and human cytomegalovirus (CMV) DNA in 688, as well as varicella zoster virus (VZV), HHV6A, HHV6B, HHV7, and Kaposi's sarcoma-associated herpesvirus (KSHV) DNA in a subset of 440 tonsil brushings. RESULTS: Overall 85% of tonsil brushing samples were infected with at least one HHV species. HHV7 and EBV were the most prevalent (≈70%), followed by HHV6B (≈50%), HSV1, CMV, VZV (≈2%), and KSHV and HSV2 (
Original languageEnglish
Pages (from-to)687-697
Number of pages11
JournalJournal of Medical Virology
Volume91
Issue number4
DOIs
Publication statusPublished - Apr 2019

Keywords

  • *epidemiology
  • *human herpesvirus
  • *oropharynx
  • *prevalence
  • *tonsil brushing

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    Kourieh, A., Gheit, T., Tommasino, M., Dalstein, V., Clifford, G. M., Lacau St Guily, J., Clavel, C., Franceschi, S., Combes, J-D., & group, SPLIT. S. (2019). Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study. Journal of Medical Virology, 91(4), 687-697. https://doi.org/10.1002/jmv.25338