Prevalence of long-QT syndrome gene variants in sudden infant death syndrome

Marianne Arnestad, Lia Crotti, Torleiv O. Rognum, Roberto Insolia, Matteo Pedrazzini, Chiara Ferrandi, Ashild Vege, Dao W. Wang, Troy E. Rhodes, Alfred L. George, Peter J. Schwartz

Research output: Contribution to journalArticle

363 Citations (Scopus)

Abstract

BACKGROUND: The hypothesis that some cases of sudden infant death syndrome (SIDS) could be caused by long-QT syndrome (LQTS) has been supported by molecular studies. However, there are inadequate data regarding the true prevalence of mutations in arrhythmia-susceptibility genes among SIDS cases. Given the importance and potential implications of these observations, we performed a study to more accurately quantify the contribution to SIDS of LQTS gene mutations and rare variants. METHODS AND RESULTS: Molecular screening of 7 genes (KCNQ1, KCNH2, SCN5A, KCNE1, KCNE2, KCNJ2, CAV3) associated with LQTS was performed with denaturing high-performance liquid chromatography and nucleotide sequencing of genomic DNA from 201 cases diagnosed as SIDS according to the Nordic Criteria, and from 182 infant and adult controls. All SIDS and control cases originated from the same regions in Norway. Genetic analysis was blinded to diagnosis. Mutations and rare variants were found in 26 of 201 cases (12.9%). On the basis of their functional effect, however, we considered 8 mutations and 7 rare variants found in 19 of 201 cases as likely contributors to sudden death (9.5%; 95% CI, 5.8 to 14.4%). CONCLUSIONS: We demonstrated that 9.5% of cases diagnosed as SIDS carry functionally significant genetic variants in LQTS genes. The present study demonstrates that sudden arrhythmic death is an important contributor to SIDS. As these variants likely modify ventricular repolarization and QT interval duration, our results support the debated concept that an ECG would probably identify most infants at risk for sudden death due to LQTS either in infancy or later on in life.

Original languageEnglish
Pages (from-to)361-367
Number of pages7
JournalCirculation
Volume115
Issue number3
DOIs
Publication statusPublished - Jan 2007

Fingerprint

Long QT Syndrome
Sudden Infant Death
Genes
Sudden Death
Mutation
Norway
DNA Sequence Analysis
Cardiac Arrhythmias
Electrocardiography
Nucleotides
High Pressure Liquid Chromatography

Keywords

  • Death, sudden
  • Genetics
  • Ion channels
  • Long-QT syndrome

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Prevalence of long-QT syndrome gene variants in sudden infant death syndrome. / Arnestad, Marianne; Crotti, Lia; Rognum, Torleiv O.; Insolia, Roberto; Pedrazzini, Matteo; Ferrandi, Chiara; Vege, Ashild; Wang, Dao W.; Rhodes, Troy E.; George, Alfred L.; Schwartz, Peter J.

In: Circulation, Vol. 115, No. 3, 01.2007, p. 361-367.

Research output: Contribution to journalArticle

Arnestad, M, Crotti, L, Rognum, TO, Insolia, R, Pedrazzini, M, Ferrandi, C, Vege, A, Wang, DW, Rhodes, TE, George, AL & Schwartz, PJ 2007, 'Prevalence of long-QT syndrome gene variants in sudden infant death syndrome', Circulation, vol. 115, no. 3, pp. 361-367. https://doi.org/10.1161/CIRCULATIONAHA.106.658021
Arnestad, Marianne ; Crotti, Lia ; Rognum, Torleiv O. ; Insolia, Roberto ; Pedrazzini, Matteo ; Ferrandi, Chiara ; Vege, Ashild ; Wang, Dao W. ; Rhodes, Troy E. ; George, Alfred L. ; Schwartz, Peter J. / Prevalence of long-QT syndrome gene variants in sudden infant death syndrome. In: Circulation. 2007 ; Vol. 115, No. 3. pp. 361-367.
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abstract = "BACKGROUND: The hypothesis that some cases of sudden infant death syndrome (SIDS) could be caused by long-QT syndrome (LQTS) has been supported by molecular studies. However, there are inadequate data regarding the true prevalence of mutations in arrhythmia-susceptibility genes among SIDS cases. Given the importance and potential implications of these observations, we performed a study to more accurately quantify the contribution to SIDS of LQTS gene mutations and rare variants. METHODS AND RESULTS: Molecular screening of 7 genes (KCNQ1, KCNH2, SCN5A, KCNE1, KCNE2, KCNJ2, CAV3) associated with LQTS was performed with denaturing high-performance liquid chromatography and nucleotide sequencing of genomic DNA from 201 cases diagnosed as SIDS according to the Nordic Criteria, and from 182 infant and adult controls. All SIDS and control cases originated from the same regions in Norway. Genetic analysis was blinded to diagnosis. Mutations and rare variants were found in 26 of 201 cases (12.9{\%}). On the basis of their functional effect, however, we considered 8 mutations and 7 rare variants found in 19 of 201 cases as likely contributors to sudden death (9.5{\%}; 95{\%} CI, 5.8 to 14.4{\%}). CONCLUSIONS: We demonstrated that 9.5{\%} of cases diagnosed as SIDS carry functionally significant genetic variants in LQTS genes. The present study demonstrates that sudden arrhythmic death is an important contributor to SIDS. As these variants likely modify ventricular repolarization and QT interval duration, our results support the debated concept that an ECG would probably identify most infants at risk for sudden death due to LQTS either in infancy or later on in life.",
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AU - Arnestad, Marianne

AU - Crotti, Lia

AU - Rognum, Torleiv O.

AU - Insolia, Roberto

AU - Pedrazzini, Matteo

AU - Ferrandi, Chiara

AU - Vege, Ashild

AU - Wang, Dao W.

AU - Rhodes, Troy E.

AU - George, Alfred L.

AU - Schwartz, Peter J.

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N2 - BACKGROUND: The hypothesis that some cases of sudden infant death syndrome (SIDS) could be caused by long-QT syndrome (LQTS) has been supported by molecular studies. However, there are inadequate data regarding the true prevalence of mutations in arrhythmia-susceptibility genes among SIDS cases. Given the importance and potential implications of these observations, we performed a study to more accurately quantify the contribution to SIDS of LQTS gene mutations and rare variants. METHODS AND RESULTS: Molecular screening of 7 genes (KCNQ1, KCNH2, SCN5A, KCNE1, KCNE2, KCNJ2, CAV3) associated with LQTS was performed with denaturing high-performance liquid chromatography and nucleotide sequencing of genomic DNA from 201 cases diagnosed as SIDS according to the Nordic Criteria, and from 182 infant and adult controls. All SIDS and control cases originated from the same regions in Norway. Genetic analysis was blinded to diagnosis. Mutations and rare variants were found in 26 of 201 cases (12.9%). On the basis of their functional effect, however, we considered 8 mutations and 7 rare variants found in 19 of 201 cases as likely contributors to sudden death (9.5%; 95% CI, 5.8 to 14.4%). CONCLUSIONS: We demonstrated that 9.5% of cases diagnosed as SIDS carry functionally significant genetic variants in LQTS genes. The present study demonstrates that sudden arrhythmic death is an important contributor to SIDS. As these variants likely modify ventricular repolarization and QT interval duration, our results support the debated concept that an ECG would probably identify most infants at risk for sudden death due to LQTS either in infancy or later on in life.

AB - BACKGROUND: The hypothesis that some cases of sudden infant death syndrome (SIDS) could be caused by long-QT syndrome (LQTS) has been supported by molecular studies. However, there are inadequate data regarding the true prevalence of mutations in arrhythmia-susceptibility genes among SIDS cases. Given the importance and potential implications of these observations, we performed a study to more accurately quantify the contribution to SIDS of LQTS gene mutations and rare variants. METHODS AND RESULTS: Molecular screening of 7 genes (KCNQ1, KCNH2, SCN5A, KCNE1, KCNE2, KCNJ2, CAV3) associated with LQTS was performed with denaturing high-performance liquid chromatography and nucleotide sequencing of genomic DNA from 201 cases diagnosed as SIDS according to the Nordic Criteria, and from 182 infant and adult controls. All SIDS and control cases originated from the same regions in Norway. Genetic analysis was blinded to diagnosis. Mutations and rare variants were found in 26 of 201 cases (12.9%). On the basis of their functional effect, however, we considered 8 mutations and 7 rare variants found in 19 of 201 cases as likely contributors to sudden death (9.5%; 95% CI, 5.8 to 14.4%). CONCLUSIONS: We demonstrated that 9.5% of cases diagnosed as SIDS carry functionally significant genetic variants in LQTS genes. The present study demonstrates that sudden arrhythmic death is an important contributor to SIDS. As these variants likely modify ventricular repolarization and QT interval duration, our results support the debated concept that an ECG would probably identify most infants at risk for sudden death due to LQTS either in infancy or later on in life.

KW - Death, sudden

KW - Genetics

KW - Ion channels

KW - Long-QT syndrome

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