Prevalence of pfcrt point mutations and level of chloroquine resistance in Plasmodium falciparum isolates from Africa

Carlo Severini, Michela Menegon, Anna Rosa Sannella, Maria Grazia Paglia, Pasquale Narciso, Alberto Matteelli, Maurizio Gulletta, Pietro Caramello, Francesca Canta, Maniphet V. Xayavong, I. N S Moura, Norman J. Pieniazek, Donatella Taramelli, Giancarlo Majori

Research output: Contribution to journalArticle

Abstract

The development in Plasmodium falciparum of the resistance to chloroquine (CQ) constitutes a public health priority, due to its direct influence in childhood mortality. The molecular basis for CQ resistance (CQR) is still unclear but, recently, a new relevant gene, named pfcrt, with several point mutations was identified in P. falciparum. Two mutations, K76T and A220S, have been considered crucial for CQR in further studies, making the pfcrt a good candidate as determinant for CQR in P. falciparum. To contribute to this topic, we have undertaken a molecular screening on 164 P. falciparum isolates from Africa: 120 isolates were Italian imported malaria cases, 27 and 17 isolates were from a school-children survey from Congo and Tanzania, respectively. In vitro tests (pLDH and WHO-Mark III tests) for CQ sensitivity have been also carried out on 28 plasmodial isolates and results compared to those obtained by molecular analysis in the same isolates. The SVIET pfcrt haplotype has been identified in the samples from Congo, and this is the first time that this haplotype is detected in Africa. Our results give further evidence to the reliability of the 76T (and the linked 74I-75E) pfcrt point mutation as molecular marker for CQR.

Original languageEnglish
Pages (from-to)262-268
Number of pages7
JournalInfection, Genetics and Evolution
Volume6
Issue number4
DOIs
Publication statusPublished - Jul 2006

Keywords

  • Chloroquine resistance
  • Molecular epidemiology
  • Molecular markers
  • pfcrt point mutations
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Microbiology
  • Infectious Diseases

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    Severini, C., Menegon, M., Sannella, A. R., Paglia, M. G., Narciso, P., Matteelli, A., Gulletta, M., Caramello, P., Canta, F., Xayavong, M. V., Moura, I. N. S., Pieniazek, N. J., Taramelli, D., & Majori, G. (2006). Prevalence of pfcrt point mutations and level of chloroquine resistance in Plasmodium falciparum isolates from Africa. Infection, Genetics and Evolution, 6(4), 262-268. https://doi.org/10.1016/j.meegid.2005.07.002