Objective: To determine type and prevalence of ndovudine resistance mutations in HIV isolated from children after long-term zidovudine treatment Methods: HIV+ children with symptomatic infection who had received at least 16 weeks of zidovudine were enrolled. Children were classified according to CDC criteria. 1994. Proviral DNA was obtained from PBMC and POL gene analyzed by nested PCR using a sequence analyzer for mutations at codon 41,67.69,70,215,219. Results: One hundred and ten children were enrolled The median age was 63 months (range 9-141 months); the median duration of zidovudine therapy was 21 months (range 471 ). Mutations were present in 93 (84.5%) children: 29 had one mutation ( 13 at codon 70. 15 at codon 215), 35 had two mutations (19 at codons 41 and 215), 18 had 3 mutations. 10 had 4 mutations and one child had 6 mutations. The number of mutations was significantly correlated with the age of the child and the duration of zidovudine treatment before study entryThe presence of mutations did not correlate with the severity of immunodeficiency. Conclusions: Zidovudine resistance mutations are highly prevalent in children who had received long-term zidovudine.The type and the prevalence of mutations are similar to those found in adult studies. Further studies are needed to clarify the clinical significance of multiple resistance mutations.
|Number of pages||1|
|Journal||Pediatric AIDS and HIV Infection|
|Publication status||Published - 1996|
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health