Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine

S. Auricchio, G. De Ritis, M. De Vincenzi, F. Latte, L. Maiuri, A. Pino, V. Raia, V. Silano

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Peptic-tryptic-cotazym and peptic-tryptic digests were obtained, simulating in vivo protein digestion, from pure 'bread' wheat gliadins and from rye, barley, and oats prolamin and tested on small intestine cultures from fetal rats. When tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and severity of degenerative changes. The ability of some sugars to interfere with inhibition of fetal intestinal morphogensis induced by these peptides was also tested. Mannan at a concentration of 0.1 mM was effective in allowing intestinal morphogenesis to take place in the presence of prolamine peptic-tryptic-cotazym and prolamine peptic-tryptic digests of the four toxic cereals. Some oligomers of N-acetylglucosamine were also effective in blocking the inhibitory effect of 'bread' wheat gliadin peptides. These data are compatible with the hypothesis that some sugars may exert a protective effect of the toxic activity of cereal prolamin peptides on the human celiac intestine.

Original languageEnglish
Pages (from-to)703-707
Number of pages5
JournalPediatric Research
Volume22
Issue number6
Publication statusPublished - 1987

Fingerprint

Prolamins
Mannans
Poisons
Abdomen
Small Intestine
Intestines
Digestion
Pancrelipase
Phenylpropanolamine
Gliadin
Peptides
Bread
Triticum
Acetylglucosamine
Hordeum
Fetal Development
Morphogenesis
Proteolysis
Culture Media
In Vitro Techniques

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Auricchio, S., De Ritis, G., De Vincenzi, M., Latte, F., Maiuri, L., Pino, A., ... Silano, V. (1987). Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine. Pediatric Research, 22(6), 703-707.

Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine. / Auricchio, S.; De Ritis, G.; De Vincenzi, M.; Latte, F.; Maiuri, L.; Pino, A.; Raia, V.; Silano, V.

In: Pediatric Research, Vol. 22, No. 6, 1987, p. 703-707.

Research output: Contribution to journalArticle

Auricchio, S, De Ritis, G, De Vincenzi, M, Latte, F, Maiuri, L, Pino, A, Raia, V & Silano, V 1987, 'Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine', Pediatric Research, vol. 22, no. 6, pp. 703-707.
Auricchio, S. ; De Ritis, G. ; De Vincenzi, M. ; Latte, F. ; Maiuri, L. ; Pino, A. ; Raia, V. ; Silano, V. / Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine. In: Pediatric Research. 1987 ; Vol. 22, No. 6. pp. 703-707.
@article{526d5b5d19d849f1968b9b3d4db52d72,
title = "Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine",
abstract = "Peptic-tryptic-cotazym and peptic-tryptic digests were obtained, simulating in vivo protein digestion, from pure 'bread' wheat gliadins and from rye, barley, and oats prolamin and tested on small intestine cultures from fetal rats. When tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and severity of degenerative changes. The ability of some sugars to interfere with inhibition of fetal intestinal morphogensis induced by these peptides was also tested. Mannan at a concentration of 0.1 mM was effective in allowing intestinal morphogenesis to take place in the presence of prolamine peptic-tryptic-cotazym and prolamine peptic-tryptic digests of the four toxic cereals. Some oligomers of N-acetylglucosamine were also effective in blocking the inhibitory effect of 'bread' wheat gliadin peptides. These data are compatible with the hypothesis that some sugars may exert a protective effect of the toxic activity of cereal prolamin peptides on the human celiac intestine.",
author = "S. Auricchio and {De Ritis}, G. and {De Vincenzi}, M. and F. Latte and L. Maiuri and A. Pino and V. Raia and V. Silano",
year = "1987",
language = "English",
volume = "22",
pages = "703--707",
journal = "Pediatric Research",
issn = "0031-3998",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - Prevention by mannan and other sugars of in vitro damage of rat fetal small intestine induced by cereal prolamin peptides toxic for human celiac intestine

AU - Auricchio, S.

AU - De Ritis, G.

AU - De Vincenzi, M.

AU - Latte, F.

AU - Maiuri, L.

AU - Pino, A.

AU - Raia, V.

AU - Silano, V.

PY - 1987

Y1 - 1987

N2 - Peptic-tryptic-cotazym and peptic-tryptic digests were obtained, simulating in vivo protein digestion, from pure 'bread' wheat gliadins and from rye, barley, and oats prolamin and tested on small intestine cultures from fetal rats. When tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and severity of degenerative changes. The ability of some sugars to interfere with inhibition of fetal intestinal morphogensis induced by these peptides was also tested. Mannan at a concentration of 0.1 mM was effective in allowing intestinal morphogenesis to take place in the presence of prolamine peptic-tryptic-cotazym and prolamine peptic-tryptic digests of the four toxic cereals. Some oligomers of N-acetylglucosamine were also effective in blocking the inhibitory effect of 'bread' wheat gliadin peptides. These data are compatible with the hypothesis that some sugars may exert a protective effect of the toxic activity of cereal prolamin peptides on the human celiac intestine.

AB - Peptic-tryptic-cotazym and peptic-tryptic digests were obtained, simulating in vivo protein digestion, from pure 'bread' wheat gliadins and from rye, barley, and oats prolamin and tested on small intestine cultures from fetal rats. When tested at a concentration of 0.1 mg of peptides/ml of culture medium the peptic-tryptic-cotazym and peptic-tryptic digests of gliadin and prolamines were very active in slowing in vitro development of fetal rat intestine and in increasing the occurrence and severity of degenerative changes. The ability of some sugars to interfere with inhibition of fetal intestinal morphogensis induced by these peptides was also tested. Mannan at a concentration of 0.1 mM was effective in allowing intestinal morphogenesis to take place in the presence of prolamine peptic-tryptic-cotazym and prolamine peptic-tryptic digests of the four toxic cereals. Some oligomers of N-acetylglucosamine were also effective in blocking the inhibitory effect of 'bread' wheat gliadin peptides. These data are compatible with the hypothesis that some sugars may exert a protective effect of the toxic activity of cereal prolamin peptides on the human celiac intestine.

UR - http://www.scopus.com/inward/record.url?scp=0023598387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023598387&partnerID=8YFLogxK

M3 - Article

VL - 22

SP - 703

EP - 707

JO - Pediatric Research

JF - Pediatric Research

SN - 0031-3998

IS - 6

ER -