Prevention by N-acetylcysteine of benzo[a]pyrene clastogenicity and DNA adducts in rats

Silvio De Flora, Francesco D'Agostini, Alberto Izzotti, Roumen Balansky

Research output: Contribution to journalArticle

Abstract

The daily i.t. administration of benzo[a]pyrene (BP) to Sprague-Dawley rats, for 3 consecutive days, did not cause any toxicity or clastogenicity in bone marrow cells, as evaluated by monitoring the ratio of polychromatic to normochromatic erythrocytes and the frequency of micronucleated polychromatic erythrocytes. However, BP produced a considerable enhancement of binucleated and micronucleated pulmonary alveolar macrophages, as well as a significant increase in polymorphonucleates recovered by bronchoalveolar lavage. These effects were prevented by administering the thiol N-acetylcysteine (NAC) by gavage 5 h before each BP instillation. In addition, the i.t. treatment with BP resulted in the formation of BP diolepoxide (BPDE)-DNA adducts in lungs and liver, as assessed by synchronous fluorescence spectrophotometry, with fluorescence peaks of similar magnitude in the 2 tissues. Pretreatment with NAC by gavage completely prevented BPDE adducts to liver DNA and significantly decreased those to lung DNA.

Original languageEnglish
Pages (from-to)87-93
Number of pages7
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume250
Issue number1-2
DOIs
Publication statusPublished - 1991

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Keywords

  • Benzo[a]pyrene
  • Bone marrow erythrocytes
  • Carcinogen-DNA adducts
  • Micronuclei
  • N-Acetylcysteine
  • Pulmonary alveolar macrophages

ASJC Scopus subject areas

  • Molecular Biology
  • Health, Toxicology and Mutagenesis

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