Prevention of spontaneous mammary adenocarcinoma in HER-2/neu transgenic mice by foreign DNA

Lucia Sfondrini, Dario Besusso, Cristiano Rumio, Monica Rodolfo, Sylvie Ménard, Andrea Balsari

Research output: Contribution to journalArticlepeer-review

Abstract

Unmethylated CpG-oligodeoxynucleotides (CpG-ODNs) are recognized as a 'danger signal' and are potent immunostimulators. To test whether tumors might be prevented by maintaining the innate immune system on continuous alert, proto-neu transgenic female mice, which develop spontaneous mammary tumors, were systemically treated with CpG-ODNs at 10-day intervals. Tumor incidence and number of tumors/mouse were significantly lower in treated mice compared with the control group. Moreover, CpG-ODN systemic treatment significantly reduced lung metastases induced by intravenous inoculation of N202.1A cells derived from a spontaneous mammary carcinoma. Growth of established tumors was modestly inhibited after CpG-ODN systemic treatment but strongly on peritumoral application. Our data indicate that systemic repeated injection of CpG-ODN to maintain the innate immune system on continuous alert prevents the onset of genetically determined tumors and confers tumor protection when the tumor load is low. - Sfondrini, L., Besusso, D., Rumio, C., Rodolfo, M., Ménard, S., Balsari, A. Prevention of spontaneous mammary adenocarcinoma in HER-2/neu transgenic mice by foreign DNA.

Original languageEnglish
Pages (from-to)1749-1754
Number of pages6
JournalFASEB Journal
Volume16
Issue number13
DOIs
Publication statusPublished - Nov 2002

Keywords

  • CpG-oligodeoxynucleotides
  • Danger signal
  • Innate immunity
  • Murine tumors

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

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