Primary HIV-1 infection sets the stage for important B lymphocyte dysfunctions

Kehmia Titanji, Francesca Chiodi, Rino Bellocco, Danika Schepis, Lyda Osorio, Chiara Tassandin, Giuseppe Tambussi, Sven Grutzmeier, Lucia Lopalco, Angelo De Milito

Research output: Contribution to journalArticle

Abstract

Objectives: To investigate the effects of primary HIV-1 infection (PHI) and of two antiretroviral therapies [highly active antiretroviral therapy (HAART) or reverse transcriptase inhibitors (RTI)] on activation, differentiation and survival of B cells. Methods: Naive and memory B cells from three groups [PHI (31), chronic infection (26) and healthy donors (12)] were studied for surface expression of Fas, LAIR-1, CD70, intracellular expression of Bcl-2 and spontaneous apoptosis. Fluorescence activated cell sorting (IgD+IgM+CD19+CD27+) and short-term cell culture to analyse induction of CD25 on B cells were performed in five patients with PHI. Patients with PHI were sampled at baseline, and after 1 and 6 months of therapy. Results were analysed by parametric and non-parametric tests and by mathematical modelling. Results: In PHI, B cells were significantly decreased; naive and memory B lymphocytes showed a high degree of activation, manifested by hypergammaglobulinaemia, altered expression of Fas and LAIR-1, and high rate of spontaneous apoptosis. Antiretroviral treatment improved the activation/differentiation status of B cells, reduced apoptosis to levels comparable to those in healthy individuals and restored the ability of B cells to respond to T cell-dependent activation. B cells showed slightly better recovery in patients taking HAART than in those taking RTI. Decreased IgM-positive memory B cells and lower induction of CD25 expression on B cells upon T cell activation at diagnosis of PHI was shown in five patients tested. These parameters normalized after 6 months of therapy. Conclusion: B cell dysfunctions found in chronic HIV-1 infection appear during PHI and initiation of antiretroviral therapy early during infection may help to preserve the B cell compartment.

Original languageEnglish
Pages (from-to)1947-1955
Number of pages9
JournalAIDS (London, England)
Volume19
Issue number17
Publication statusPublished - Nov 2005

Keywords

  • Antiretroviral therapy
  • B cells
  • Immune activation
  • Primary HIV infection

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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  • Cite this

    Titanji, K., Chiodi, F., Bellocco, R., Schepis, D., Osorio, L., Tassandin, C., Tambussi, G., Grutzmeier, S., Lopalco, L., & De Milito, A. (2005). Primary HIV-1 infection sets the stage for important B lymphocyte dysfunctions. AIDS (London, England), 19(17), 1947-1955.