Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes

Luciano Mutti, Maria T. Valle, Bruno Balbi, Anna M. Orengo, Antonio Lazzaro, Paolo Alciato, Emiliano Gatti, Pier G. Betta, Ernesto Pozzi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Mesothelioma cells (MMc) are considered to be weakly immunogenic and the experimental approaches attempting to induce an immune response against these cells have been disappointing. Our aim was to investigate whether MMC possess the surface accessory molecules involved in antigen presentation and whether these cells are capable of presenting recall antigens to autologous blood lymphocytes. Four primary MMc cultures were generated from malignant effusions and examined to assess whether the accessory molecules required for antigen presentation were present on their surfaces. Intercellular adhesion molecule-I (ICAM-I; CD54); class I and class II major histocompatibility complex-DR (MHCI and MHCII-DR); B7-1 (CD80.3); and B7-2 (CD86) expression by MMc was studied by immunocytochemical and/or FACScan analysis. MMc were pulsed with purified protein derivative (PPD), Tetanus toxoid (TT) and Candida albicans (CA) bodies, and incubated with autologous lymphocytes. Lymphocyte proliferation was estimated by radionucleotide incorporation. Phenotypic analysis showed the presence of MHCII-DR, ICAM-1 and B7-2 on primary MMc cultures, whereas the phenotypic evaluation of 2 established MMc lines did not show the presence of the B7-1 and B7-2 molecules. In addition, MHCII-DR was detectable only after interferon gamma (IFN-γ) stimulation. Primary MMc cultures acquired the capability to induce lymphocyte proliferation after pulse with the recall antigens. To achieve characterization of these lymphocytes, we generated a PPD-specific CD4+ T- cell clone. PPD-pulsed MMc were shown to specifically induce T-cell clone proliferation through a MHCII-DR-mediated process. We conclude that primary MMC possess the surface molecules required for antigen presentation and can present recall antigens to CD4+ lymphocytes.

Original languageEnglish
Pages (from-to)740-749
Number of pages10
JournalInternational Journal of Cancer
Volume78
Issue number6
Publication statusPublished - 1998

Fingerprint

Mesothelioma
Autoantigens
Intercellular Adhesion Molecule-1
Lymphocytes
Primary Cell Culture
Antigen Presentation
Clone Cells
B7 Antigens
T-Lymphocytes
CD4 Antigens
Tetanus Toxoid
Proteins
Cell Adhesion Molecules
Major Histocompatibility Complex
Candida albicans
Interferon-gamma
Cell Proliferation
Antigens
Cell Line

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes. / Mutti, Luciano; Valle, Maria T.; Balbi, Bruno; Orengo, Anna M.; Lazzaro, Antonio; Alciato, Paolo; Gatti, Emiliano; Betta, Pier G.; Pozzi, Ernesto.

In: International Journal of Cancer, Vol. 78, No. 6, 1998, p. 740-749.

Research output: Contribution to journalArticle

Mutti, L, Valle, MT, Balbi, B, Orengo, AM, Lazzaro, A, Alciato, P, Gatti, E, Betta, PG & Pozzi, E 1998, 'Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes', International Journal of Cancer, vol. 78, no. 6, pp. 740-749.
Mutti, Luciano ; Valle, Maria T. ; Balbi, Bruno ; Orengo, Anna M. ; Lazzaro, Antonio ; Alciato, Paolo ; Gatti, Emiliano ; Betta, Pier G. ; Pozzi, Ernesto. / Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes. In: International Journal of Cancer. 1998 ; Vol. 78, No. 6. pp. 740-749.
@article{0a50cc62228547e59ba904fd04e3f6cb,
title = "Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes",
abstract = "Mesothelioma cells (MMc) are considered to be weakly immunogenic and the experimental approaches attempting to induce an immune response against these cells have been disappointing. Our aim was to investigate whether MMC possess the surface accessory molecules involved in antigen presentation and whether these cells are capable of presenting recall antigens to autologous blood lymphocytes. Four primary MMc cultures were generated from malignant effusions and examined to assess whether the accessory molecules required for antigen presentation were present on their surfaces. Intercellular adhesion molecule-I (ICAM-I; CD54); class I and class II major histocompatibility complex-DR (MHCI and MHCII-DR); B7-1 (CD80.3); and B7-2 (CD86) expression by MMc was studied by immunocytochemical and/or FACScan analysis. MMc were pulsed with purified protein derivative (PPD), Tetanus toxoid (TT) and Candida albicans (CA) bodies, and incubated with autologous lymphocytes. Lymphocyte proliferation was estimated by radionucleotide incorporation. Phenotypic analysis showed the presence of MHCII-DR, ICAM-1 and B7-2 on primary MMc cultures, whereas the phenotypic evaluation of 2 established MMc lines did not show the presence of the B7-1 and B7-2 molecules. In addition, MHCII-DR was detectable only after interferon gamma (IFN-γ) stimulation. Primary MMc cultures acquired the capability to induce lymphocyte proliferation after pulse with the recall antigens. To achieve characterization of these lymphocytes, we generated a PPD-specific CD4+ T- cell clone. PPD-pulsed MMc were shown to specifically induce T-cell clone proliferation through a MHCII-DR-mediated process. We conclude that primary MMC possess the surface molecules required for antigen presentation and can present recall antigens to CD4+ lymphocytes.",
author = "Luciano Mutti and Valle, {Maria T.} and Bruno Balbi and Orengo, {Anna M.} and Antonio Lazzaro and Paolo Alciato and Emiliano Gatti and Betta, {Pier G.} and Ernesto Pozzi",
year = "1998",
language = "English",
volume = "78",
pages = "740--749",
journal = "International Journal of Cancer",
issn = "0020-7136",
publisher = "Wiley-Liss Inc.",
number = "6",

}

TY - JOUR

T1 - Primary human mesothelioma cells express class II MHC, ICAM-1 and B7-2 and can present recall antigens to autologous blood lymphocytes

AU - Mutti, Luciano

AU - Valle, Maria T.

AU - Balbi, Bruno

AU - Orengo, Anna M.

AU - Lazzaro, Antonio

AU - Alciato, Paolo

AU - Gatti, Emiliano

AU - Betta, Pier G.

AU - Pozzi, Ernesto

PY - 1998

Y1 - 1998

N2 - Mesothelioma cells (MMc) are considered to be weakly immunogenic and the experimental approaches attempting to induce an immune response against these cells have been disappointing. Our aim was to investigate whether MMC possess the surface accessory molecules involved in antigen presentation and whether these cells are capable of presenting recall antigens to autologous blood lymphocytes. Four primary MMc cultures were generated from malignant effusions and examined to assess whether the accessory molecules required for antigen presentation were present on their surfaces. Intercellular adhesion molecule-I (ICAM-I; CD54); class I and class II major histocompatibility complex-DR (MHCI and MHCII-DR); B7-1 (CD80.3); and B7-2 (CD86) expression by MMc was studied by immunocytochemical and/or FACScan analysis. MMc were pulsed with purified protein derivative (PPD), Tetanus toxoid (TT) and Candida albicans (CA) bodies, and incubated with autologous lymphocytes. Lymphocyte proliferation was estimated by radionucleotide incorporation. Phenotypic analysis showed the presence of MHCII-DR, ICAM-1 and B7-2 on primary MMc cultures, whereas the phenotypic evaluation of 2 established MMc lines did not show the presence of the B7-1 and B7-2 molecules. In addition, MHCII-DR was detectable only after interferon gamma (IFN-γ) stimulation. Primary MMc cultures acquired the capability to induce lymphocyte proliferation after pulse with the recall antigens. To achieve characterization of these lymphocytes, we generated a PPD-specific CD4+ T- cell clone. PPD-pulsed MMc were shown to specifically induce T-cell clone proliferation through a MHCII-DR-mediated process. We conclude that primary MMC possess the surface molecules required for antigen presentation and can present recall antigens to CD4+ lymphocytes.

AB - Mesothelioma cells (MMc) are considered to be weakly immunogenic and the experimental approaches attempting to induce an immune response against these cells have been disappointing. Our aim was to investigate whether MMC possess the surface accessory molecules involved in antigen presentation and whether these cells are capable of presenting recall antigens to autologous blood lymphocytes. Four primary MMc cultures were generated from malignant effusions and examined to assess whether the accessory molecules required for antigen presentation were present on their surfaces. Intercellular adhesion molecule-I (ICAM-I; CD54); class I and class II major histocompatibility complex-DR (MHCI and MHCII-DR); B7-1 (CD80.3); and B7-2 (CD86) expression by MMc was studied by immunocytochemical and/or FACScan analysis. MMc were pulsed with purified protein derivative (PPD), Tetanus toxoid (TT) and Candida albicans (CA) bodies, and incubated with autologous lymphocytes. Lymphocyte proliferation was estimated by radionucleotide incorporation. Phenotypic analysis showed the presence of MHCII-DR, ICAM-1 and B7-2 on primary MMc cultures, whereas the phenotypic evaluation of 2 established MMc lines did not show the presence of the B7-1 and B7-2 molecules. In addition, MHCII-DR was detectable only after interferon gamma (IFN-γ) stimulation. Primary MMc cultures acquired the capability to induce lymphocyte proliferation after pulse with the recall antigens. To achieve characterization of these lymphocytes, we generated a PPD-specific CD4+ T- cell clone. PPD-pulsed MMc were shown to specifically induce T-cell clone proliferation through a MHCII-DR-mediated process. We conclude that primary MMC possess the surface molecules required for antigen presentation and can present recall antigens to CD4+ lymphocytes.

UR - http://www.scopus.com/inward/record.url?scp=0031771249&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031771249&partnerID=8YFLogxK

M3 - Article

C2 - 9833768

AN - SCOPUS:0031771249

VL - 78

SP - 740

EP - 749

JO - International Journal of Cancer

JF - International Journal of Cancer

SN - 0020-7136

IS - 6

ER -