Primary laminopathy fibroblasts display altered genome organization and apoptosis

Karen J. Meaburn, Erik Cabuy, Gisele Bonne, Nicolas Levy, Glenn E. Morris, Giuseppe Novelli, Ian R. Kill, Joanna M. Bridger

Research output: Contribution to journalArticle

Abstract

A number of diseases associated with specific tissue degeneration and premature aging have mutations in the nuclear envelope proteins A-type lamins or emerin. Those diseases with A-type lamin mutation are inclusively termed laminopathies. Due to various hypothetical roles of nuclear envelope proteins in genome function we investigated whether alterations to normal genomic behaviour are apparent in cells with mutations in A-type lamins and emerin. Even though the distributions of these proteins in proliferating laminopathy fibroblasts appear normal, there is abnormal nuclear positioning of both chromosome 18 and 13 territories, from the nuclear periphery to the interior. This genomic organization mimics that found in normal nonproliferating quiescent or senescent cells. This finding is supported by distributions of modified pRb in the laminopathy cells. All laminopathy cell lines tested and an X-linked Emery-Dreifuss muscular dystrophy cell line also demonstrate increased incidences of apoptosis. The most extreme cases of apoptosis occur in cells derived from diseases with mutations in the tail region of the LMNA gene, such as Dunningan-type familial partial lipodystrophy and mandibuloacral dysplasia, and this correlates with a significant level of micronucleation in these cells.

Original languageEnglish
Pages (from-to)139-153
Number of pages15
JournalAging Cell
Volume6
Issue number2
DOIs
Publication statusPublished - Apr 2007

Keywords

  • A-type lamins
  • Apoptosis
  • Genome organization
  • Laminopathies
  • Micronucleation
  • Nuclear architecture
  • Quiescence
  • Senescence

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Meaburn, K. J., Cabuy, E., Bonne, G., Levy, N., Morris, G. E., Novelli, G., Kill, I. R., & Bridger, J. M. (2007). Primary laminopathy fibroblasts display altered genome organization and apoptosis. Aging Cell, 6(2), 139-153. https://doi.org/10.1111/j.1474-9726.2007.00270.x