Primary structure of α-globin chains from river buffalo (bubalus bubalis L.) hemoglobins

Pasquale Ferranti, Angelo Facchiano, Francesca Zappacosta, Donatella Vincenti, Rosario Rullo, Bruno Masala, Aldo Di Luccia

Research output: Contribution to journalArticle

Abstract

Primary structure analysis of the four river buffalo α-globin chains showed that haplotypes A and B differ from each other by a substitution at codon 64 that may encode Ala or Asn. The A haplotype encodes two α-globin chains, Iα1 and IIα3, which differ at positions 129 and 131: Iα1 has 64 Ala, 129 Phe, 131 Asn; IIα3 has 64 Ala, 129 Leu, 131 Ser. The B haplotype encodes two α-globin chains, Iα2 and IIα4, which differ at positions 10 and 11: Iα2 has 10 Ile, 11 Gin, 64 Asn; IIα4 has 10 Val, 11 Lys, 64 Asn. Apart from the Ala/Asn polymorphism at position 64, amino acid substitutions in allelic and nonallelic α-globin chains seem to have arisen by single point mutations. Detection of electrophoretically silent mutations due to neutral amino acid substitutions and their influence on the isoelectric point are discussed. Furthermore, primary structures of river buffalo α-globin chains are compared to other species of the Bovidae family to suggest evolutionary events that have characterized the amino acid substitutions of river buffalo hemoglobin.

Original languageEnglish
Pages (from-to)171-179
Number of pages9
JournalProtein Journal
Volume20
Issue number2
Publication statusPublished - 2001

Keywords

  • α-globin chain
  • Mass spectrometry
  • Molecular modeling
  • Primary structure
  • River buffalo hemoglobin

ASJC Scopus subject areas

  • Biochemistry

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    Ferranti, P., Facchiano, A., Zappacosta, F., Vincenti, D., Rullo, R., Masala, B., & Di Luccia, A. (2001). Primary structure of α-globin chains from river buffalo (bubalus bubalis L.) hemoglobins. Protein Journal, 20(2), 171-179.