TY - JOUR
T1 - Prime-boost vaccination with attenuated Salmonella Typhimurium (increment)znuABC and inactivated Salmonella Choleraesuis is protective against Salmonella Choleraesuis challenge infection in piglets
AU - Alborali, Giovanni Loris
AU - Ruggeri, Jessica
AU - Pesciaroli, Michele
AU - Martinelli, Nicola
AU - Chirullo, Barbara
AU - Ammendola, Serena
AU - Battistoni, Andrea
AU - Ossiprandi, Maria Cristina
AU - Corradi, Attilio
AU - Pasquali, Paolo
PY - 2017/9/11
Y1 - 2017/9/11
N2 - Background: Salmonella enterica serovar Choleraesuis (S. Choleraesuis) infection causes a systemic disease in pigs. Vaccination could represent a solution to reduce prevalence in farms. In this study, we aimed to assess the efficacy of an attenuated strain of Salmonella enterica serovar Typhimurium (S. Typhimurium (increment)znuABC) against S. Choleraesuis infection. The vaccination protocol combined priming with attenuated S. Typhimurium (increment)znuABC vaccine and boost with an inactivated S. Choleraesuis vaccine and we compared the protection conferred to that induced by an inactivated S. Choleraesuis vaccine. Methods: The first group of piglets was orally vaccinated with S. Typhimurium (increment)znuABC and boosted with inactivated S. Choleraesuis, the second one was intramuscularly vaccinated with S. Choleraesuis inactivated vaccine and the third group of piglets was unvaccinated. All groups of animals were challenged with a virulent S. Choleraesuis strain at day 35 post vaccination. Results: The results showed that the vaccination protocol, priming with S. Typhimurium (increment)znuABC and boosted with inactivated S. Choleraesuis, applied to group A was able to limit weight loss, fever and organs colonization, arising from infection with virulent S. Choleraesuis, more effectively, than the prime-boost vaccination with homologous S. Choleraesuis inactivated vaccine (group B). Conclusion: In conclusion, these research findings extend the validity of attenuated S. Typhimurium (increment)znuABC strain as a useful mucosal vaccine against S. Typhimurium and S. Choleraesuis pig infection. The development of combined vaccination protocols can have a diffuse administration in field conditions because animals are generally infected with different concomitant serovars.
AB - Background: Salmonella enterica serovar Choleraesuis (S. Choleraesuis) infection causes a systemic disease in pigs. Vaccination could represent a solution to reduce prevalence in farms. In this study, we aimed to assess the efficacy of an attenuated strain of Salmonella enterica serovar Typhimurium (S. Typhimurium (increment)znuABC) against S. Choleraesuis infection. The vaccination protocol combined priming with attenuated S. Typhimurium (increment)znuABC vaccine and boost with an inactivated S. Choleraesuis vaccine and we compared the protection conferred to that induced by an inactivated S. Choleraesuis vaccine. Methods: The first group of piglets was orally vaccinated with S. Typhimurium (increment)znuABC and boosted with inactivated S. Choleraesuis, the second one was intramuscularly vaccinated with S. Choleraesuis inactivated vaccine and the third group of piglets was unvaccinated. All groups of animals were challenged with a virulent S. Choleraesuis strain at day 35 post vaccination. Results: The results showed that the vaccination protocol, priming with S. Typhimurium (increment)znuABC and boosted with inactivated S. Choleraesuis, applied to group A was able to limit weight loss, fever and organs colonization, arising from infection with virulent S. Choleraesuis, more effectively, than the prime-boost vaccination with homologous S. Choleraesuis inactivated vaccine (group B). Conclusion: In conclusion, these research findings extend the validity of attenuated S. Typhimurium (increment)znuABC strain as a useful mucosal vaccine against S. Typhimurium and S. Choleraesuis pig infection. The development of combined vaccination protocols can have a diffuse administration in field conditions because animals are generally infected with different concomitant serovars.
KW - Attenuated and inactivated vaccines
KW - IFN-gamma
KW - Mucosal vaccine
KW - Piglets
KW - Salmonella Choleraesuis
KW - Salmonellosis
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U2 - 10.1186/s12917-017-1202-5
DO - 10.1186/s12917-017-1202-5
M3 - Article
C2 - 28893256
AN - SCOPUS:85029232092
VL - 13
JO - BMC Veterinary Research
JF - BMC Veterinary Research
SN - 1746-6148
IS - 1
M1 - 284
ER -