Priming and inducing effects of interleukin-3 on histamine release from cord-blood basophils

A. Miadonna, C. Salmaso, M. P. Di Marco, L. Pugni, N. Milazzo, A. Tedeschi, A. Marini

Research output: Contribution to journalArticle

Abstract

The effects of interleukin-3 (IL-3) on histamine release from cord and adult blood basophils were evaluated. Leukocyte suspensions, obtained from adult patients with respiratory allergy (n = 15), normal adult subjects (n= 15), and neonates with (n=15) and without (n=19) atopic disposition, were stimulated with anti-IgE, fMLP, and IL-3. IgE-mediated histamine release was significantly higher in adult patients, either allergic or normal, than in neonates with or without atopic disposition. A trend toward higher fMLP- induced histamine release was found in allergic adult subjects. IL-3 had a weak direct histamine-releasing activity in allergic adult subjects and in neonates, but not in normal adult donors. A significant enhancing effect of IL-3 on histamine release induced by anti-IgE was observed in neonates with and without atopic disposition and in normal adult subjects, but not in atopic adult patients. IL-3 exerted a priming effect also when basophils were stimulated with fMLP, without any significant difference between neonates and adult subjects. Passive sensitization with IgE-rich serum resulted in a significant increase in anti-IgE-induced, but not in IL-3-induced, histamine release from cord-blood basophils. In conclusion, IL-3 primes cord-blood as well as adult blood basophils for a consecutive anti-IgE- or fMLP-induced histamine release, and its activity is not limited by the low density of membrane IgE in cord-blood basophils.

Original languageEnglish
Pages (from-to)992-998
Number of pages7
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume52
Issue number10
Publication statusPublished - 1997

Keywords

  • Atopic disposition
  • Basophils
  • Cord blood
  • Histamine release
  • Interleukin-3

ASJC Scopus subject areas

  • Immunology

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    Miadonna, A., Salmaso, C., Di Marco, M. P., Pugni, L., Milazzo, N., Tedeschi, A., & Marini, A. (1997). Priming and inducing effects of interleukin-3 on histamine release from cord-blood basophils. Allergy: European Journal of Allergy and Clinical Immunology, 52(10), 992-998.