PRL-3 phosphatase is implicated in ovarian cancer growth

Federica Polato, Annamaria Codegoni, Robert Fruscio, Patrizia Perego, Costantino Mangioni, Saurabh Saha, Alberto Bardelli, Massimo Broggini

Research output: Contribution to journalArticle

Abstract

Purpose: The PRL-3 phosphatase has been found expressed at higher levels in metastasis than in primary tumors of patients with colorectal cancer. In the present study, we evaluated the expression of PRL-3 in ovarian cancer tissue and its role in ovarian cancer cell growth. Experimental Design: PRL-3 phosphatase expression was evaluated in 84 ovarian tumor samples. PRL-3 expression has been knocked down using specific small interfering RNAs to determine its role in ovarian cancer cell growth in vitro. Results: In ovarian cancers, PRL-3 expression correlates with disease progression, being higher in advanced (stage III) than in early (stage I) tumors. In situ measurements of PRL-3 expression showed that it was confined to the epithelial neoplastic celts. The molecular mechanism underlying PRL-3 overexpression in ovarian cancers is independent from amplification of the corresponding genomic locus. Ovarian cancer cells growing in culture have high levels of expression of this phosphatase. PRL-3-specific knockdown using small interfering RNA severely impaired the growth of cells without affecting the expression of the closely related homologue PRL-1. Intriguingly, the growth of human colon carcinoma cells expressing lower levels of the PRL-3 was not affected by the PRL-3 knockdown. Conclusions: Altogether, these results show that PRL-3 expression is associated with ovarian cancer progression and point to a key role for this phosphatase in the control of ovarian cancer cells growth. This strongly suggests that PRL-3 should be considered as a target for the discovery of new anticancer agents to be tested against this malignancy.

Original languageEnglish
Pages (from-to)6835-6839
Number of pages5
JournalClinical Cancer Research
Volume11
Issue number19 I
DOIs
Publication statusPublished - Oct 1 2005

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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  • Cite this

    Polato, F., Codegoni, A., Fruscio, R., Perego, P., Mangioni, C., Saha, S., Bardelli, A., & Broggini, M. (2005). PRL-3 phosphatase is implicated in ovarian cancer growth. Clinical Cancer Research, 11(19 I), 6835-6839. https://doi.org/10.1158/1078-0432.CCR-04-2357