Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection: Interactions influence outcome

Carlo F. Zambon, Daniela Basso, Filippo Navaglia, Claudio Belluco, Alessandra Falda, Paola Fogar, Eliana Greco, Nicoletta Gallo, Massimo Rugge, Francesco Di Mario, Mario Plebani

Research output: Contribution to journalArticle

Abstract

The aim of this study was to evaluate whether there was any correlation between Helicobacter pylori-associated diseases and (1) H. pylori virulence genes or (2) IL-1B, IL-1RN, IFN-G, TNF-A, IL-10 genetic polymorphisms. Patients with non-cardia gastric cancer (NCGC, n = 129) or benign gastroduodenal diseases (n = 792) were studied. IL-1RN intron 2 VNTR polymorphism (PCR), IL-1B -31 C/T (RFLP), the SNPs of IFN-G (+874 A/T), TNF-A (-1031 C/T, -857 C/T, -376 A/G, -308 A/G, -238 A/G), IL-10 (-1082 A/G, -819 C/T, -592 A/C) (Taqman chemistry) were studied. cagA, s1 and m1 vacA, were PCR amplified. Duodenal ulcer was more frequent in TNF-A -857 TT and in IL-1RN 1,2 subjects. TNF-A -857 TT genotype was also correlated with gastric ulcer. IL-10 -819 TT genotype was associated with intestinal metaplasia and NCGC. Antral inflammation was associated with TNF-A -1031 TT, while corpus activity with IL-10 -819 CC. H. pylori infection was associated with TNF-A -308 AG genotype, while IFN-G +874 AA genotype was associated with cagA. In conclusion, among host genetic factors contributing to H. pylori disease outcome, IFN-G +874 AA genotype favors cagA positive infections, TNF-A -857 TT duodenal ulcer while IL-10 -819 TT intestinal metaplasia and NCGC.

Original languageEnglish
Pages (from-to)141-152
Number of pages12
JournalCytokine
Volume29
Issue number4
DOIs
Publication statusPublished - Feb 21 2005

Fingerprint

Helicobacter Infections
Polymorphism
Helicobacter pylori
Interleukin-10
Anti-Inflammatory Agents
Genes
Genotype
Cytokines
Metaplasia
Duodenal Ulcer
Polymerase Chain Reaction
Genetic Polymorphisms
Stomach Ulcer
Interleukin-1
Restriction Fragment Length Polymorphisms
Introns
Stomach Neoplasms
Interleukin-2
Single Nucleotide Polymorphism
Virulence

Keywords

  • Gastric cancer
  • Helicobacter pylori
  • Interleukin-1 alpha
  • Interleukin-10
  • Tumor necrosis factor alpha

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection : Interactions influence outcome. / Zambon, Carlo F.; Basso, Daniela; Navaglia, Filippo; Belluco, Claudio; Falda, Alessandra; Fogar, Paola; Greco, Eliana; Gallo, Nicoletta; Rugge, Massimo; Di Mario, Francesco; Plebani, Mario.

In: Cytokine, Vol. 29, No. 4, 21.02.2005, p. 141-152.

Research output: Contribution to journalArticle

Zambon, CF, Basso, D, Navaglia, F, Belluco, C, Falda, A, Fogar, P, Greco, E, Gallo, N, Rugge, M, Di Mario, F & Plebani, M 2005, 'Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection: Interactions influence outcome', Cytokine, vol. 29, no. 4, pp. 141-152. https://doi.org/10.1016/j.cyto.2004.10.013
Zambon, Carlo F. ; Basso, Daniela ; Navaglia, Filippo ; Belluco, Claudio ; Falda, Alessandra ; Fogar, Paola ; Greco, Eliana ; Gallo, Nicoletta ; Rugge, Massimo ; Di Mario, Francesco ; Plebani, Mario. / Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection : Interactions influence outcome. In: Cytokine. 2005 ; Vol. 29, No. 4. pp. 141-152.
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AU - Belluco, Claudio

AU - Falda, Alessandra

AU - Fogar, Paola

AU - Greco, Eliana

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AU - Di Mario, Francesco

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