TY - JOUR
T1 - Pro- and anti-inflammatory cytokines gene polymorphisms and Helicobacter pylori infection
T2 - Interactions influence outcome
AU - Zambon, Carlo F.
AU - Basso, Daniela
AU - Navaglia, Filippo
AU - Belluco, Claudio
AU - Falda, Alessandra
AU - Fogar, Paola
AU - Greco, Eliana
AU - Gallo, Nicoletta
AU - Rugge, Massimo
AU - Di Mario, Francesco
AU - Plebani, Mario
PY - 2005/2/21
Y1 - 2005/2/21
N2 - The aim of this study was to evaluate whether there was any correlation between Helicobacter pylori-associated diseases and (1) H. pylori virulence genes or (2) IL-1B, IL-1RN, IFN-G, TNF-A, IL-10 genetic polymorphisms. Patients with non-cardia gastric cancer (NCGC, n = 129) or benign gastroduodenal diseases (n = 792) were studied. IL-1RN intron 2 VNTR polymorphism (PCR), IL-1B -31 C/T (RFLP), the SNPs of IFN-G (+874 A/T), TNF-A (-1031 C/T, -857 C/T, -376 A/G, -308 A/G, -238 A/G), IL-10 (-1082 A/G, -819 C/T, -592 A/C) (Taqman chemistry) were studied. cagA, s1 and m1 vacA, were PCR amplified. Duodenal ulcer was more frequent in TNF-A -857 TT and in IL-1RN 1,2 subjects. TNF-A -857 TT genotype was also correlated with gastric ulcer. IL-10 -819 TT genotype was associated with intestinal metaplasia and NCGC. Antral inflammation was associated with TNF-A -1031 TT, while corpus activity with IL-10 -819 CC. H. pylori infection was associated with TNF-A -308 AG genotype, while IFN-G +874 AA genotype was associated with cagA. In conclusion, among host genetic factors contributing to H. pylori disease outcome, IFN-G +874 AA genotype favors cagA positive infections, TNF-A -857 TT duodenal ulcer while IL-10 -819 TT intestinal metaplasia and NCGC.
AB - The aim of this study was to evaluate whether there was any correlation between Helicobacter pylori-associated diseases and (1) H. pylori virulence genes or (2) IL-1B, IL-1RN, IFN-G, TNF-A, IL-10 genetic polymorphisms. Patients with non-cardia gastric cancer (NCGC, n = 129) or benign gastroduodenal diseases (n = 792) were studied. IL-1RN intron 2 VNTR polymorphism (PCR), IL-1B -31 C/T (RFLP), the SNPs of IFN-G (+874 A/T), TNF-A (-1031 C/T, -857 C/T, -376 A/G, -308 A/G, -238 A/G), IL-10 (-1082 A/G, -819 C/T, -592 A/C) (Taqman chemistry) were studied. cagA, s1 and m1 vacA, were PCR amplified. Duodenal ulcer was more frequent in TNF-A -857 TT and in IL-1RN 1,2 subjects. TNF-A -857 TT genotype was also correlated with gastric ulcer. IL-10 -819 TT genotype was associated with intestinal metaplasia and NCGC. Antral inflammation was associated with TNF-A -1031 TT, while corpus activity with IL-10 -819 CC. H. pylori infection was associated with TNF-A -308 AG genotype, while IFN-G +874 AA genotype was associated with cagA. In conclusion, among host genetic factors contributing to H. pylori disease outcome, IFN-G +874 AA genotype favors cagA positive infections, TNF-A -857 TT duodenal ulcer while IL-10 -819 TT intestinal metaplasia and NCGC.
KW - Gastric cancer
KW - Helicobacter pylori
KW - Interleukin-1 alpha
KW - Interleukin-10
KW - Tumor necrosis factor alpha
UR - http://www.scopus.com/inward/record.url?scp=19944433955&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19944433955&partnerID=8YFLogxK
U2 - 10.1016/j.cyto.2004.10.013
DO - 10.1016/j.cyto.2004.10.013
M3 - Article
C2 - 15652446
AN - SCOPUS:19944433955
VL - 29
SP - 141
EP - 152
JO - Cytokine
JF - Cytokine
SN - 1043-4666
IS - 4
ER -