Abstract
Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.
| Original language | English |
|---|---|
| Pages (from-to) | 721-728 |
| Number of pages | 8 |
| Journal | Journal of Cystic Fibrosis |
| Volume | 12 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2013 |
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Keywords
- Cystic fibrosis
- Lipopolysaccharide
- Lung inflammation
- Macrophages
- Neutrophils
- Primary ciliary dyskinesia
ASJC Scopus subject areas
- Pulmonary and Respiratory Medicine
- Pediatrics, Perinatology, and Child Health
Cite this
Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung. / Porro, Chiara; Di Gioia, Sante; Trotta, Teresa; Lepore, Silvia; Panaro, Maria Antonietta; Battaglino, Anna; Ratclif, Luigi; Castellani, Stefano; Bufo, Pantaleo; Martinez, Maria Carmen; Conese, Massimo.
In: Journal of Cystic Fibrosis, Vol. 12, No. 6, 12.2013, p. 721-728.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung
AU - Porro, Chiara
AU - Di Gioia, Sante
AU - Trotta, Teresa
AU - Lepore, Silvia
AU - Panaro, Maria Antonietta
AU - Battaglino, Anna
AU - Ratclif, Luigi
AU - Castellani, Stefano
AU - Bufo, Pantaleo
AU - Martinez, Maria Carmen
AU - Conese, Massimo
PY - 2013/12
Y1 - 2013/12
N2 - Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.
AB - Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.
KW - Cystic fibrosis
KW - Lipopolysaccharide
KW - Lung inflammation
KW - Macrophages
KW - Neutrophils
KW - Primary ciliary dyskinesia
UR - http://www.scopus.com/inward/record.url?scp=84888055209&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84888055209&partnerID=8YFLogxK
U2 - 10.1016/j.jcf.2013.03.002
DO - 10.1016/j.jcf.2013.03.002
M3 - Article
C2 - 23567201
AN - SCOPUS:84888055209
VL - 12
SP - 721
EP - 728
JO - Journal of Cystic Fibrosis
JF - Journal of Cystic Fibrosis
SN - 1569-1993
IS - 6
ER -