Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung

Chiara Porro, Sante Di Gioia, Teresa Trotta, Silvia Lepore, Maria Antonietta Panaro, Anna Battaglino, Luigi Ratclif, Stefano Castellani, Pantaleo Bufo, Maria Carmen Martinez, Massimo Conese

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.

Original languageEnglish
Pages (from-to)721-728
Number of pages8
JournalJournal of Cystic Fibrosis
Volume12
Issue number6
DOIs
Publication statusPublished - Dec 2013

Fingerprint

Sputum
Cystic Fibrosis
Lipopolysaccharides
Lung
Neutrophils
Inflammation
Horseshoe Crabs
Acute Lung Injury
Bronchoalveolar Lavage Fluid
Granulocytes
Cell Count
Macrophages
Tissue Donors

Keywords

  • Cystic fibrosis
  • Lipopolysaccharide
  • Lung inflammation
  • Macrophages
  • Neutrophils
  • Primary ciliary dyskinesia

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Pediatrics, Perinatology, and Child Health

Cite this

Porro, C., Di Gioia, S., Trotta, T., Lepore, S., Panaro, M. A., Battaglino, A., ... Conese, M. (2013). Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung. Journal of Cystic Fibrosis, 12(6), 721-728. https://doi.org/10.1016/j.jcf.2013.03.002

Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung. / Porro, Chiara; Di Gioia, Sante; Trotta, Teresa; Lepore, Silvia; Panaro, Maria Antonietta; Battaglino, Anna; Ratclif, Luigi; Castellani, Stefano; Bufo, Pantaleo; Martinez, Maria Carmen; Conese, Massimo.

In: Journal of Cystic Fibrosis, Vol. 12, No. 6, 12.2013, p. 721-728.

Research output: Contribution to journalArticle

Porro, C, Di Gioia, S, Trotta, T, Lepore, S, Panaro, MA, Battaglino, A, Ratclif, L, Castellani, S, Bufo, P, Martinez, MC & Conese, M 2013, 'Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung', Journal of Cystic Fibrosis, vol. 12, no. 6, pp. 721-728. https://doi.org/10.1016/j.jcf.2013.03.002
Porro C, Di Gioia S, Trotta T, Lepore S, Panaro MA, Battaglino A et al. Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung. Journal of Cystic Fibrosis. 2013 Dec;12(6):721-728. https://doi.org/10.1016/j.jcf.2013.03.002
Porro, Chiara ; Di Gioia, Sante ; Trotta, Teresa ; Lepore, Silvia ; Panaro, Maria Antonietta ; Battaglino, Anna ; Ratclif, Luigi ; Castellani, Stefano ; Bufo, Pantaleo ; Martinez, Maria Carmen ; Conese, Massimo. / Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung. In: Journal of Cystic Fibrosis. 2013 ; Vol. 12, No. 6. pp. 721-728.
@article{c15fa935c1304ba1b6fd35f2f7b59a7a,
title = "Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung",
abstract = "Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.",
keywords = "Cystic fibrosis, Lipopolysaccharide, Lung inflammation, Macrophages, Neutrophils, Primary ciliary dyskinesia",
author = "Chiara Porro and {Di Gioia}, Sante and Teresa Trotta and Silvia Lepore and Panaro, {Maria Antonietta} and Anna Battaglino and Luigi Ratclif and Stefano Castellani and Pantaleo Bufo and Martinez, {Maria Carmen} and Massimo Conese",
year = "2013",
month = "12",
doi = "10.1016/j.jcf.2013.03.002",
language = "English",
volume = "12",
pages = "721--728",
journal = "Journal of Cystic Fibrosis",
issn = "1569-1993",
publisher = "Elsevier",
number = "6",

}

TY - JOUR

T1 - Pro-inflammatory effect of cystic fibrosis sputum microparticles in the murine lung

AU - Porro, Chiara

AU - Di Gioia, Sante

AU - Trotta, Teresa

AU - Lepore, Silvia

AU - Panaro, Maria Antonietta

AU - Battaglino, Anna

AU - Ratclif, Luigi

AU - Castellani, Stefano

AU - Bufo, Pantaleo

AU - Martinez, Maria Carmen

AU - Conese, Massimo

PY - 2013/12

Y1 - 2013/12

N2 - Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.

AB - Background: The role of microparticles (MPs) in the inflammatory process of cystic fibrosis (CF) airways is not known. Here, we have studied the pro-inflammatory potential of CF MPs in a model of acute lung injury. Methods: Swiss mice were subjected to intratracheal administration of MPs obtained from CF and primary ciliary diskinesia (PCD) patients. Histopathology, total and differential cell counts in bronchoalveolar lavage fluid were used to evaluate the inflammatory reaction in the lung. Lipopolysaccharide (LPS)-like activity of MPs was studied by Limulus amebocyte lysate assay. Results: MPs obtained from acute CF patients determined peribronchial and perivascular inflammatory infiltrates similar to those elicited by LPS. This inflammation was granulocyte-dominated and higher than that determined by MPs obtained from stable CF, whereas PCD MPs caused a macrophage-dominated inflammation. While LPS-activity was not found in circulating blood MPs prepared from CF patients, it was present in MPs obtained from CF sputum and sputum CD66b+ neutrophils. Finally, LPS-like activity was only detected in circulating MPs after incubation with LPS as well as in MPs obtained from LPS-stimulated neutrophils obtained from healthy donors. Conclusions: These data suggest that the pro-inflammatory potential of neutrophil-derived MPs in the CF airways may be subsequent to the binding of shedded LPS.

KW - Cystic fibrosis

KW - Lipopolysaccharide

KW - Lung inflammation

KW - Macrophages

KW - Neutrophils

KW - Primary ciliary dyskinesia

UR - http://www.scopus.com/inward/record.url?scp=84888055209&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888055209&partnerID=8YFLogxK

U2 - 10.1016/j.jcf.2013.03.002

DO - 10.1016/j.jcf.2013.03.002

M3 - Article

VL - 12

SP - 721

EP - 728

JO - Journal of Cystic Fibrosis

JF - Journal of Cystic Fibrosis

SN - 1569-1993

IS - 6

ER -