Procedure for the purification of the fibronection proteolytic fragments containing the ED-B oncofetal domain

Laura Borsi, Enrica Balza, Alessandra Leprini, Marco Ponassi, Luciano Zardi

Research output: Contribution to journalArticle

Abstract

Fibronectin (FN) is the blend of structurally different molecules (isoforms) whose makeup varies depending on the FN sources. Fibronectin polymorphism is caused by three sequences (called ED-A, ED-B, and IIICS) which may be included or excluded from the FN molecule depending on the alternative splicing patterns of a single primary transcript. The sequence ED-B, which is a complete type III repeat of 91 amino acids, presents some interesting peculiarities: it is the most conserved FN region and, in normal adult tissues, the ED-B-containing FN has an extremely restricted distribution while having a much greater expression in fetal and tumor tissues (Carnemolla et al., 1989, J. Cell Biol. 108, 1139-1148), suggesting that the ED-B sequence may confer to the FN molecules specific biological activities required during ontogenesis and oncogenetic processes. Here we describe a detailed procedure to purify fibronectin fragments containing the ED-B sequence. These purified fragments are useful reagents in the study of the biological function(s) of the ED-B-containing FN molecules.

Original languageEnglish
Pages (from-to)372-379
Number of pages8
JournalAnalytical Biochemistry
Volume192
Issue number2
DOIs
Publication statusPublished - Feb 1 1991

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Fibronectins
Purification
Molecules
Tissue
Alternative Splicing
Bioactivity
Polymorphism
Tumors
Protein Isoforms
Fetus
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Procedure for the purification of the fibronection proteolytic fragments containing the ED-B oncofetal domain. / Borsi, Laura; Balza, Enrica; Leprini, Alessandra; Ponassi, Marco; Zardi, Luciano.

In: Analytical Biochemistry, Vol. 192, No. 2, 01.02.1991, p. 372-379.

Research output: Contribution to journalArticle

Borsi, Laura ; Balza, Enrica ; Leprini, Alessandra ; Ponassi, Marco ; Zardi, Luciano. / Procedure for the purification of the fibronection proteolytic fragments containing the ED-B oncofetal domain. In: Analytical Biochemistry. 1991 ; Vol. 192, No. 2. pp. 372-379.
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