Procedure for the quantitation of Gadd45 expression levels in clonal hematopoietic progenitor cells by competitive polymerase chain reaction

Maria Alessandra Santucci, Alessandro Ripalti, Maria Chiara Di Paola, Anna Maria Mianulli, Enza Iacurti, Fabio Campanini, Barbara Gamberi, Sante Tura

Research output: Contribution to journalArticle

Abstract

Objective: The growth arrest and DNA damage-inducible (gadd) genes represent a family of stress-inducible genes that are coordinately regulated at transcriptional level. Gadd45, in particular, has been linked to a p53- dependent inducible network required for regulated transition from G1 to S phase of cell cycle following genotoxic insult and growth arrest treatments and has seemingly a pivotal role in DNA repair. Design and methods: Here we show that competitive polymerase chain reaction (PCR) is an adequate method to quantitate gadd45 expression levels in hematopoietic progenitor cell line 32D, whose constitutive gene expression is very low. Results: The sensitivity and reproducibility of our strategy support its usefulness for clinical purposes, to assess the DNA repair capacity of highly purified early myeloid progenitors, whose failure may be responsible for either short-term chemotherapy side effects (bone marrow hypoplasia and peripheral blood cytopenia) or long-term consequences of antiblastic drugs (leukemia and myelodysplasia).

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalClinical Biochemistry
Volume32
Issue number1
DOIs
Publication statusPublished - Feb 1999

Keywords

  • Competitive PCR
  • Gadd45
  • Hematopoiesis

ASJC Scopus subject areas

  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Procedure for the quantitation of Gadd45 expression levels in clonal hematopoietic progenitor cells by competitive polymerase chain reaction'. Together they form a unique fingerprint.

  • Cite this