Process of differentiation of cerebellar Purkinje neurons in the chick embryo

Mirella Bertossi, Luisa Roncali, Lucia Mancini, Domenico Ribatti, Beatrice Nico

Research output: Contribution to journalArticle

Abstract

The microscopic and ultrastructural differentiation of Purkinje neurons has been studied in 40 chicken embryo cerebella, from the 10th incubation day to hatching, and the transverse diameter of the cell body measured, for each developmental stage, on 30 electron micrographs of sagittally cut Purkinje cells. The developing Purkinje cell bodies, bipolar, at first, given the presence of two processes emerging from the opposite poles of the oval perikaryon, grow progressively in size. After the 12th incubation day, they develop a branched dendritic tree, and, shortly before hatching time, the cells acquire the characteristic flask or pear-shaped configuration. On the 10th incubation day, microtubules are already detectable together with Golgi complexes and a few vesicles of rough endoplasmic reticulum; on the 14th incubation day, RER cisterns are recognizable in the supranuclear cytoplasm, later extending into the whole perikaryon, and attaining their definitive distribution by the 18th incubation day. Pinocytotic and coated vesicles, as well as subsurface cisterns are seen during the whole embryonic life. In the earliest stages of development, three distinct types of junctional contacts between Purkinje cells and surrounding axons are described, and their functional role in relation to synaptogenetic processes is discussed. Beginning with the 16th incubation day, some Purkinje neurons undergo degenerative changes similar to those described in other types of neurons of the central and peripheral nervous system.

Original languageEnglish
Pages (from-to)25-34
Number of pages10
JournalAnatomy and Embryology
Volume175
Issue number1
DOIs
Publication statusPublished - Nov 1986

Fingerprint

Purkinje Cells
Chick Embryo
Coated Vesicles
Pyrus
Rough Endoplasmic Reticulum
Peripheral Nervous System
Golgi Apparatus
Microtubules
Cerebellum
Axons
Chickens
Cytoplasm
Embryonic Structures
Central Nervous System
Electrons
Neurons
Cell Body

Keywords

  • Cerebellar cortex
  • Chick embryo
  • Neuronal degeneration
  • Purkinje cell differentiation

ASJC Scopus subject areas

  • Embryology
  • Anatomy
  • Developmental Biology
  • Cell Biology

Cite this

Bertossi, M., Roncali, L., Mancini, L., Ribatti, D., & Nico, B. (1986). Process of differentiation of cerebellar Purkinje neurons in the chick embryo. Anatomy and Embryology, 175(1), 25-34. https://doi.org/10.1007/BF00315453

Process of differentiation of cerebellar Purkinje neurons in the chick embryo. / Bertossi, Mirella; Roncali, Luisa; Mancini, Lucia; Ribatti, Domenico; Nico, Beatrice.

In: Anatomy and Embryology, Vol. 175, No. 1, 11.1986, p. 25-34.

Research output: Contribution to journalArticle

Bertossi, M, Roncali, L, Mancini, L, Ribatti, D & Nico, B 1986, 'Process of differentiation of cerebellar Purkinje neurons in the chick embryo', Anatomy and Embryology, vol. 175, no. 1, pp. 25-34. https://doi.org/10.1007/BF00315453
Bertossi, Mirella ; Roncali, Luisa ; Mancini, Lucia ; Ribatti, Domenico ; Nico, Beatrice. / Process of differentiation of cerebellar Purkinje neurons in the chick embryo. In: Anatomy and Embryology. 1986 ; Vol. 175, No. 1. pp. 25-34.
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