Abstract
The primary transcriptional product of eukaryotic tRNA genes is a precursor molecule with extrancleotides at the 5' and 3' end. We show that the 5' and 3' sequences, uniquely present in the RNA precursor molecule do not play any role in the efficiency and accuracy of processing reactions. If, however, as a consequence of in vitro manipulation, these extranucleotides from a base-paired extension of the aminoacid acceptor stem, the rate of processing is slowed down. The rate of processing is brought back to normal in a single base-pair deletion mutant probably as a consequence of a destabilization of the base-paired extension of the aminoacid acceptor stem.
Original language | English |
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Pages (from-to) | 4135-4145 |
Number of pages | 11 |
Journal | Nucleic Acids Research |
Volume | 10 |
Issue number | 14 |
DOIs | |
Publication status | Published - Jul 24 1982 |
ASJC Scopus subject areas
- Statistics, Probability and Uncertainty
- Applied Mathematics
- Health, Toxicology and Mutagenesis
- Toxicology
- Genetics(clinical)
- Genetics