Processing of eukaryotic tRNA precursors: Secondary structure of the precursor specific sequences affects the rate but not the accuracy of processing reactions

Luisa Castangnoli, Gennaro Ciliberto, Riccardo Cortese

Research output: Contribution to journalArticlepeer-review

Abstract

The primary transcriptional product of eukaryotic tRNA genes is a precursor molecule with extrancleotides at the 5' and 3' end. We show that the 5' and 3' sequences, uniquely present in the RNA precursor molecule do not play any role in the efficiency and accuracy of processing reactions. If, however, as a consequence of in vitro manipulation, these extranucleotides from a base-paired extension of the aminoacid acceptor stem, the rate of processing is slowed down. The rate of processing is brought back to normal in a single base-pair deletion mutant probably as a consequence of a destabilization of the base-paired extension of the aminoacid acceptor stem.

Original languageEnglish
Pages (from-to)4135-4145
Number of pages11
JournalNucleic Acids Research
Volume10
Issue number14
DOIs
Publication statusPublished - Jul 24 1982

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

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