Abstract
The presence of fibrin deposits in the micro environment of tumor cells has been reported repeatedly and considered to play an important role in tumor biology. Among the mechanisms by which fibrin may be deposited in tumors, procoagulant activities (PCA) of different types have been described in cancer cells. The present study was aimed at establishing whether the nature of cellular PCA was a characteristic associated with malignant transformation. PCA of normal and transformed cells was investigated on pairs of murine and human origin. The transformed counterparts were obtained after treatment with low‐dose radiation, chemical carcinogen, viral infection or after in vitro spontaneous immortalization. Both before and after any type of transformation cell PCA was of the tissue thromboplastin type, identified on the basis of biological criteria: requirement of factor VII for its expression and lack of inhibition by the serine protease inhibitor diisopropylfluorophosphate (DFP). Transformed cells of murine origin showed significantly lower activity than their normal counterparts, whereas all the transformed human cell lines expressed significantly higher activity than normal. An inverse correlation between the levels of PCA and the cell density in culture was observed in all but one of the lines tested. These findings suggest that the factor X activating property described in some tumors or in transformed cells cannot be considered as a general marker of transformation.
| Original language | English |
|---|---|
| Pages (from-to) | 411-414 |
| Number of pages | 4 |
| Journal | International Journal of Cancer |
| Volume | 35 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 1985 |
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ASJC Scopus subject areas
- Medicine(all)
- Oncology
- Cancer Research
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Procoagulant activity of mouse and human cultured cells following various types of transformation. / Curatolo, Laura; Alessio, Grazia; Passerini, Carlo Gambacorti; Casali, Bruno; Morasca, Luciano; Semeraro, Nicola; Donati, Maria Benedetta.
In: International Journal of Cancer, Vol. 35, No. 3, 1985, p. 411-414.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Procoagulant activity of mouse and human cultured cells following various types of transformation
AU - Curatolo, Laura
AU - Alessio, Grazia
AU - Passerini, Carlo Gambacorti
AU - Casali, Bruno
AU - Morasca, Luciano
AU - Semeraro, Nicola
AU - Donati, Maria Benedetta
PY - 1985
Y1 - 1985
N2 - The presence of fibrin deposits in the micro environment of tumor cells has been reported repeatedly and considered to play an important role in tumor biology. Among the mechanisms by which fibrin may be deposited in tumors, procoagulant activities (PCA) of different types have been described in cancer cells. The present study was aimed at establishing whether the nature of cellular PCA was a characteristic associated with malignant transformation. PCA of normal and transformed cells was investigated on pairs of murine and human origin. The transformed counterparts were obtained after treatment with low‐dose radiation, chemical carcinogen, viral infection or after in vitro spontaneous immortalization. Both before and after any type of transformation cell PCA was of the tissue thromboplastin type, identified on the basis of biological criteria: requirement of factor VII for its expression and lack of inhibition by the serine protease inhibitor diisopropylfluorophosphate (DFP). Transformed cells of murine origin showed significantly lower activity than their normal counterparts, whereas all the transformed human cell lines expressed significantly higher activity than normal. An inverse correlation between the levels of PCA and the cell density in culture was observed in all but one of the lines tested. These findings suggest that the factor X activating property described in some tumors or in transformed cells cannot be considered as a general marker of transformation.
AB - The presence of fibrin deposits in the micro environment of tumor cells has been reported repeatedly and considered to play an important role in tumor biology. Among the mechanisms by which fibrin may be deposited in tumors, procoagulant activities (PCA) of different types have been described in cancer cells. The present study was aimed at establishing whether the nature of cellular PCA was a characteristic associated with malignant transformation. PCA of normal and transformed cells was investigated on pairs of murine and human origin. The transformed counterparts were obtained after treatment with low‐dose radiation, chemical carcinogen, viral infection or after in vitro spontaneous immortalization. Both before and after any type of transformation cell PCA was of the tissue thromboplastin type, identified on the basis of biological criteria: requirement of factor VII for its expression and lack of inhibition by the serine protease inhibitor diisopropylfluorophosphate (DFP). Transformed cells of murine origin showed significantly lower activity than their normal counterparts, whereas all the transformed human cell lines expressed significantly higher activity than normal. An inverse correlation between the levels of PCA and the cell density in culture was observed in all but one of the lines tested. These findings suggest that the factor X activating property described in some tumors or in transformed cells cannot be considered as a general marker of transformation.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022259535&partnerID=8YFLogxK
U2 - 10.1002/ijc.2910350318
DO - 10.1002/ijc.2910350318
M3 - Article
C2 - 3972474
AN - SCOPUS:0022259535
VL - 35
SP - 411
EP - 414
JO - International Journal of Cancer
JF - International Journal of Cancer
SN - 0020-7136
IS - 3
ER -