Abstract
Newly synthesized procollagen type I (PC) assembles into 300 nm rigid, rod-like triple helices in the lumen of the endoplasmic reticulum. This oligomeric complex moves to the Golgi and forms large electron-dense aggregates. We have monitored the transport of PC along the secretory pathway. We show that PC moves across the Golgi stacks without ever leaving the lumen of the Golgi cisternae. During transport from the endoplasmic reticulum to the Golgi, PC is found within tubular-saccular structures greater than 300 nm in length. Thus, supermolecular cargoes such as PC do not utilize the conventional vesicle-mediated transport to traverse the Golgi stacks. Our results imply that PC moves in the anterograde direction across the Golgi complex by a process involving progressive maturation of Golgi cisternae.
| Original language | English |
|---|---|
| Pages (from-to) | 993-1003 |
| Number of pages | 11 |
| Journal | Cell |
| Volume | 95 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Dec 23 1998 |
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ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
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Procollagen traverses the Golgi stack without leaving the lumen of cisternae : Evidence for cisternal maturation. / Bonfanti, Lidia; Mironov, Alexander A.; Martínez-Menárguez, José A.; Martella, Oliviano; Fusella, Aurora; Baldassarre, Massimiliano; Buccione, Roberto; Geuze, Hans J.; Mironov, Alexander A.; Luini, Alberto.
In: Cell, Vol. 95, No. 7, 23.12.1998, p. 993-1003.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Procollagen traverses the Golgi stack without leaving the lumen of cisternae
T2 - Evidence for cisternal maturation
AU - Bonfanti, Lidia
AU - Mironov, Alexander A.
AU - Martínez-Menárguez, José A.
AU - Martella, Oliviano
AU - Fusella, Aurora
AU - Baldassarre, Massimiliano
AU - Buccione, Roberto
AU - Geuze, Hans J.
AU - Mironov, Alexander A.
AU - Luini, Alberto
PY - 1998/12/23
Y1 - 1998/12/23
N2 - Newly synthesized procollagen type I (PC) assembles into 300 nm rigid, rod-like triple helices in the lumen of the endoplasmic reticulum. This oligomeric complex moves to the Golgi and forms large electron-dense aggregates. We have monitored the transport of PC along the secretory pathway. We show that PC moves across the Golgi stacks without ever leaving the lumen of the Golgi cisternae. During transport from the endoplasmic reticulum to the Golgi, PC is found within tubular-saccular structures greater than 300 nm in length. Thus, supermolecular cargoes such as PC do not utilize the conventional vesicle-mediated transport to traverse the Golgi stacks. Our results imply that PC moves in the anterograde direction across the Golgi complex by a process involving progressive maturation of Golgi cisternae.
AB - Newly synthesized procollagen type I (PC) assembles into 300 nm rigid, rod-like triple helices in the lumen of the endoplasmic reticulum. This oligomeric complex moves to the Golgi and forms large electron-dense aggregates. We have monitored the transport of PC along the secretory pathway. We show that PC moves across the Golgi stacks without ever leaving the lumen of the Golgi cisternae. During transport from the endoplasmic reticulum to the Golgi, PC is found within tubular-saccular structures greater than 300 nm in length. Thus, supermolecular cargoes such as PC do not utilize the conventional vesicle-mediated transport to traverse the Golgi stacks. Our results imply that PC moves in the anterograde direction across the Golgi complex by a process involving progressive maturation of Golgi cisternae.
UR - http://www.scopus.com/inward/record.url?scp=0032417711&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032417711&partnerID=8YFLogxK
U2 - 10.1016/S0092-8674(00)81723-7
DO - 10.1016/S0092-8674(00)81723-7
M3 - Article
C2 - 9875853
AN - SCOPUS:0032417711
VL - 95
SP - 993
EP - 1003
JO - Cell
JF - Cell
SN - 0092-8674
IS - 7
ER -