Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells

M. L. Nolli, E. Sarubbi, A. Corti, F. Robbiati, A. Soflientini, F. Blasi, F. Parenti, G. Cassani

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Human chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalFibrinolysis and Proteolysis
Volume3
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Urokinase-Type Plasminogen Activator
Fibrinolysin
Bioreactors
Immunoblotting
Clone Cells
DNA

Keywords

  • Fibrinolysis
  • Mammalian cell culture
  • Recombinant DNA
  • Single chain urokinase-type plasminogen activator

ASJC Scopus subject areas

  • Hematology

Cite this

Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells. / Nolli, M. L.; Sarubbi, E.; Corti, A.; Robbiati, F.; Soflientini, A.; Blasi, F.; Parenti, F.; Cassani, G.

In: Fibrinolysis and Proteolysis, Vol. 3, No. 2, 1989, p. 101-106.

Research output: Contribution to journalArticle

Nolli, M. L. ; Sarubbi, E. ; Corti, A. ; Robbiati, F. ; Soflientini, A. ; Blasi, F. ; Parenti, F. ; Cassani, G. / Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells. In: Fibrinolysis and Proteolysis. 1989 ; Vol. 3, No. 2. pp. 101-106.
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AU - Parenti, F.

AU - Cassani, G.

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