Production and characterisation of human recombinant single chain urokinase-type plasminogen activator from mouse cells

M. L. Nolli, E. Sarubbi, A. Corti, F. Robbiati, A. Soflientini, F. Blasi, F. Parenti, G. Cassani

Research output: Contribution to journalArticle

Abstract

Human chromosomal DNA coding for single chain urokinase-type plasminogen activator (scu-PA) has been inserted into a vector and expressed in LB6 cells. The recombinant clone termed 1C9 is stable and produces scu-PA at about 0.8 mg/I/day in a bioreactor. The scu-PA produced is human by amino- and carboxy-terminal analysis and immunoblotting. The recombinant scu-PA is glycosylated and fully activatable by plasmin.

Original languageEnglish
Pages (from-to)101-106
Number of pages6
JournalFibrinolysis and Proteolysis
Volume3
Issue number2
DOIs
Publication statusPublished - 1989

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Keywords

  • Fibrinolysis
  • Mammalian cell culture
  • Recombinant DNA
  • Single chain urokinase-type plasminogen activator

ASJC Scopus subject areas

  • Hematology

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