Production and characterization of a human recombinant monoclonal Fab fragment specific for influenza a viruses

Alessandra Desogus, Roberto Burioni, Angela Ingianni, Francesca Bugli, Raffaello Pompei, Giovanni Fadda

Research output: Contribution to journalArticlepeer-review

Abstract

A human recombinant monoclonal Fab fragment that specifically recognizes all the influenza A virus strains tested was produced in transformed Escherichia coli using the phage display technique. No strain of influenza B virus reacted with it. It was purified after four cycles of panning and by a single passage through an immunoaffinity column. About 1 mg of pure monoclonal antibody was obtained from 1 liter of culture medium in 3 working days. The Fab fragment reacted with a viral 27-kDa protein, which could reasonably be a matrix protein. Indirect immunofluorescence tests performed on virus-infected MDCK cells showed that this Fab fragment was at least equally efficient as other commercial monoclonal antibody-based systems in detecting influenza A viral infections. The potential advantages of human recombinant Fabs on murine monoclonal antibodies are discussed.

Original languageEnglish
Pages (from-to)680-685
Number of pages6
JournalClinical and Diagnostic Laboratory Immunology
Volume10
Issue number4
DOIs
Publication statusPublished - Jul 2003

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

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