TY - JOUR
T1 - Production and characterization of antibody probes directed at constant regions of the α and the β subunit of the human T cell receptor
AU - Fabbi, M.
AU - Acuto, O.
AU - Bensussan, A.
AU - Poole, C. B.
AU - Reinherz, E. L.
PY - 1985
Y1 - 1985
N2 - To generate antibodies directed at constant regions of the human T cell receptor, purified α and β subunits of a human T cell antigen/major histocompatibility complex receptor from the REX tumor (Ti-REX) were isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and utilized to immunize rabbits. H36 (anti-α subunit) and H38 (anti-β subunit) antisera were strongly reactive with the denatured subunits and also immunoprecipitated the Ti heterodimer from 125I surface-labeled lysates of REX, inducer, suppressor and cytotoxic T cell clones, peripheral T lymphocytes and thymocytes. Moreover, immunodepletion experiments showed that such antisera recognized antigenic determinant(s) shared by all Ti molecules expressed in the thymus. Several observations were made with these anticonstant region antibodies. First, peptide map analysis showed that the T cell receptor molecules recognized by the anti-clonotype and the anti-constant region heteroantisera on a given T cell clone are identical, thus supporting the view that the T cell receptor undergoes allelic exclusion. Second, since the individual antisera were weakly cross-reactive with the other denatured subunit, such subunits probably share conserved sequences. Third, the absence of antisera reactivity with intact cells implies that most of these constant region epitopes must be obscured by associated molecules, perhaps including one or more of the 20-25 kDa T3 subunits. Fourth, the extensive difference in two-dimensional peptide maps of Ti α subunits from clones of differing specificities makes it likely that the subunit contributes in a major way to antigen/ major histocompatibility complex binding.
AB - To generate antibodies directed at constant regions of the human T cell receptor, purified α and β subunits of a human T cell antigen/major histocompatibility complex receptor from the REX tumor (Ti-REX) were isolated by preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis and utilized to immunize rabbits. H36 (anti-α subunit) and H38 (anti-β subunit) antisera were strongly reactive with the denatured subunits and also immunoprecipitated the Ti heterodimer from 125I surface-labeled lysates of REX, inducer, suppressor and cytotoxic T cell clones, peripheral T lymphocytes and thymocytes. Moreover, immunodepletion experiments showed that such antisera recognized antigenic determinant(s) shared by all Ti molecules expressed in the thymus. Several observations were made with these anticonstant region antibodies. First, peptide map analysis showed that the T cell receptor molecules recognized by the anti-clonotype and the anti-constant region heteroantisera on a given T cell clone are identical, thus supporting the view that the T cell receptor undergoes allelic exclusion. Second, since the individual antisera were weakly cross-reactive with the other denatured subunit, such subunits probably share conserved sequences. Third, the absence of antisera reactivity with intact cells implies that most of these constant region epitopes must be obscured by associated molecules, perhaps including one or more of the 20-25 kDa T3 subunits. Fourth, the extensive difference in two-dimensional peptide maps of Ti α subunits from clones of differing specificities makes it likely that the subunit contributes in a major way to antigen/ major histocompatibility complex binding.
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U2 - 10.1002/eji.1830150815
DO - 10.1002/eji.1830150815
M3 - Article
C2 - 2411571
AN - SCOPUS:0022378469
VL - 15
SP - 821
EP - 827
JO - European Journal of Immunology
JF - European Journal of Immunology
SN - 0014-2980
IS - 8
ER -