Production and characterization of monoclonal antibodies directed against the laminin receptor precursor

S. Butò, C. Ghirelli, P. Aiello, E. Tagliabue, E. Ardini, A. Magnifico, N. Montuori, M. E. Sobel, M. I. Colnaghi, S. Ménard

Research output: Contribution to journalArticlepeer-review

Abstract

The 67-kDa laminin receptor (67LR) is an important tumor marker whose molecular structure has not yet been fully elucidated. To shed new light on this molecule, we raised a series of eight new monoclonal antibodies, designated MPLR1 to 8, directed against the 37-kDa recombinant laminin receptor precursor (37LRP). Cross-competition experiments demonstrated that the epitopes recognized by MPLR2, 4 and 5 partially overlap, since MPLR4 and 5 compete with labelled MPLR2 for the binding to recombinant 37LRP. These three antibodies belong to the IgG1 class, whereas the other ones are all IgM. Presumably due to the fact that they are directed against partially unfolded antigenic determinants expressed on the recombinant protein, MPLRs did not recognize the native protein. Indeed, they showed no reactivity at the membrane level in cytofluorimetric analysis and they did not work in immunoprecipitation experiments. In contrast, these reagents are valuable tools in immunoblotting, since they clearly identify a 67-kDa protein (the mature laminin receptor) in addition to the 37-kDa precursor form. MPLRs are thus a new powerful tool which could help in the characterization of the still enigmatic 67LR molecule.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalInternational Journal of Biological Markers
Volume12
Issue number1
Publication statusPublished - Jan 1997

Keywords

  • 37LRP monoclonal antibodies
  • 67-kDa laminin receptor
  • 67LR 37-kDa precursor of the laminin receptor
  • MAbs

ASJC Scopus subject areas

  • Immunology
  • Biochemistry

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