Production and characterization of two immunotoxins specific for M5b ANLL leukaemia

R. Tecce, R. Fraioli, P. De Fabritiis, A. Sandrelli, A. Savarese, L. Santoro, M. Cuomo, P. G. Natali

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Abstract

We describe the production of functional characterization of 2 monocytic-cell-lineage-specific immunotoxins constructed with saporin emitoxin (SP) from Saponaria officinalis. Interest in the production of these immunotoxins, of possible clinical relevance, has been raised by the availability of 2 MAbs of high specificity for circulating monocytes and M5b ANLL, thus envisaging their potential use in bone-marrow purging. SAP emitoxin was selected on the basis of the low cytotoxicity in unconjugated form, as opposed to highly specific cytotoxicity and favourable pharmacokinetical properties in the conjugated form. SPDP conjugation produced immunotoxins which retained serological specificity and protein-synthesis-inhibitory activity. The 2 immunotoxins did not interfere with bone-marrow progenitor-cell growth in a CFU-GM colony assay. On the contrary, they were capable of killing monocytic cells selectively, as demonstrated in phenotypical and functional assays. Thus these 2 novel immunotoxins appear to be promising reagents in purging autologous bone marrow prior to transplantation in patients suffering from monocytic leukaemia.

Original languageEnglish
Pages (from-to)310-316
Number of pages7
JournalInternational Journal of Cancer
Volume49
Issue number2
Publication statusPublished - 1991

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Tecce, R., Fraioli, R., De Fabritiis, P., Sandrelli, A., Savarese, A., Santoro, L., Cuomo, M., & Natali, P. G. (1991). Production and characterization of two immunotoxins specific for M5b ANLL leukaemia. International Journal of Cancer, 49(2), 310-316.