We evaluated the production and the secretion of interleukin (IL)-1α, IL-1β and IL-6 in three human malignant melanoma cell lines, TVM-BO, SK-Mel 28 and Bowes. The results indicate the heterogeneity of IL production among the lines investigated, and that IL-6 is the most actively produced and secreted cytokine. IL-1, both α and β, are produced at much lower rates and are mainly cytoplasmic. Moreover we modified the growth rate of the cell lines by exposure to 5 μM retinoic acid or 5 mM α-difluoromethylornithine, known to reduce proliferation by about 30% and 90% respectively, over untreated cultures. IL-6 production and secretion was significantly affected by both treatments, suggesting that also for malignant melanoma cell lines, IL-6 may act as an autocrine growth factor to control proliferation.
|Number of pages||4|
|Journal||Clinical Chemistry and Enzymology Communications|
|Publication status||Published - 1993|
- Retinoic acid
ASJC Scopus subject areas
- Clinical Biochemistry