Production and secretion of interleukins 1 and 6 by melanoma tumors in vitro upon retinoic acid or α-difluoromethylornithine exposure

P. Riches, R. Gooding, D. Eustace, P. Guerrieri, M. Annicchiarico-Petruzzelli, L. R. Tartaglia, G. Melino

Research output: Contribution to journalArticlepeer-review

Abstract

We evaluated the production and the secretion of interleukin (IL)-1α, IL-1β and IL-6 in three human malignant melanoma cell lines, TVM-BO, SK-Mel 28 and Bowes. The results indicate the heterogeneity of IL production among the lines investigated, and that IL-6 is the most actively produced and secreted cytokine. IL-1, both α and β, are produced at much lower rates and are mainly cytoplasmic. Moreover we modified the growth rate of the cell lines by exposure to 5 μM retinoic acid or 5 mM α-difluoromethylornithine, known to reduce proliferation by about 30% and 90% respectively, over untreated cultures. IL-6 production and secretion was significantly affected by both treatments, suggesting that also for malignant melanoma cell lines, IL-6 may act as an autocrine growth factor to control proliferation.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalClinical Chemistry and Enzymology Communications
Volume6
Issue number1-2
Publication statusPublished - 1993

Keywords

  • α-difluoromethylornithine
  • Interleukin-1
  • Interleukin-6
  • Melanoma
  • Retinoic acid

ASJC Scopus subject areas

  • Clinical Biochemistry

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