Production of a monoclonal antibody directed against the recombinant SEL1L protein

R. Orlandi, M. Cattaneo, F. Troglio, M. Campiglio, I. Biunno, Sylvie Ménard

Research output: Contribution to journalArticlepeer-review

Abstract

SEL1L, highly similar to the C. elegans sel-1 gene, is a recently cloned human gene whose function is under investigation. SEL1L is differentially expressed in tumors and normal tissues and seems to play a role in tumor growth and aggressiveness. We used the recombinant N-terminus of the SEL1L protein to immunize a Balb/c mouse and produce a monoclonal antibody. A hybridoma secreting an antibody specifically reacting on the SEL1L recombinant fragment was selected. This monoclonal antibody, named MSel1, recognizes the SEL1L protein by Western blotting, immunofluorescence and immunohistochemistry on normal and tumor cells. MSel1 is able to recognize SEL1L even on archival tumor specimens and is therefore particularly appropriate to study SEL1L involvement in tumor progression.

Original languageEnglish
Pages (from-to)104-111
Number of pages8
JournalInternational Journal of Biological Markers
Volume17
Issue number2
Publication statusPublished - 2002

Keywords

  • Lung carcinoma
  • Monoclonal antibody
  • SEL1L

ASJC Scopus subject areas

  • Biochemistry
  • Immunology

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