Production of B cell growth factor by a Leu-7±, OKM1+ non-T cell with the features of large granular lymphocytes (LGL)1

V. Pistoia, F. Cozzolino, M. Torcia, E. Castigli, M. Ferrarini

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Abstract

The present study reports the characterization of a non-T cell from human peripheral blood which is capable of releasing BCGF. This BCGF-producing non-T cell had a T3-, T8-, Leu-7+, OKM1+, HLA-DR- Leu-11- surface phenotype and was likely to belong to the so-called large granular lymphocyte (LGL) subset because: 1) after fractionation of non-T cells according to the expression of Leu-7 or HLA-DR markers, it was found in the Leu-7+, HLA-DR- fractions that were particularly enriched in LGL; 2) it co-purified with LGL on Percoll density gradients; and 3) it expressed Leu-7 and OKM1 markers that are shared by a large fraction of LGL. Although copurified with cells with potent NK capacities, the BCGF-producing cell was not cytotoxic, because treatment of Leu-7+ cells with Leu-11 monoclonal antibody and complement abolished the NK activity but left the BCGF activity unaltered. The factor released by this LGL subset was not IL 1 or IL 2 mistakenly interpreted as BCGF, because: a) cell supernatants particularly rich in BCGF activity contained very little or no IL 1 or IL 2; b) BCGF-induced B cell proliferation was not inhibitable by anti-Tac antibodies (this in spite of the expression of IL 2 receptor by a proportion of activated B cells); and c) BCGF activity was absorbed by B but not T blasts.

Original languageEnglish
Pages (from-to)3179-3184
Number of pages6
JournalJournal of Immunology
Volume134
Issue number5
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Immunology

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    Pistoia, V., Cozzolino, F., Torcia, M., Castigli, E., & Ferrarini, M. (1985). Production of B cell growth factor by a Leu-7±, OKM1+ non-T cell with the features of large granular lymphocytes (LGL)1 . Journal of Immunology, 134(5), 3179-3184.