Production of colony stimulating factor by EL-4 lymphoma cells stimulated with phytohemoagglutinin

Augusto Pessina, Paolo Brambilla, Maria Grazia Neri, Paolo Mocarelli

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Murine EL-4 lymphoma cells when stimulated with optimal doses of phytohemoagglutinin (PHA) release colony stimulating factor (CSF) in the conditioned medium. CSF production depends on PHA and stops when the lectin is withdrawn. The production is dependent on protein synthesis and is not related with DNA replication. The CSF produced by EL-4 cells stimulates murine bone marrow cells to differentiate in vitro into mature granulocytes. Unstimulated EL-4 cells do not produce CSF while its conditioned medium is able to inhibit EL-4 proliferation in vitro. Gel filtration chromatography of conditioned medium from PHA-stimulated EL4 cells yields a peak of activity corresponding to an apparent molecular weight of 20,000 - 25,000 daltons.

Original languageEnglish
Pages (from-to)549-553
Number of pages5
JournalInternational Journal of Immunopharmacology
Volume7
Issue number4
DOIs
Publication statusPublished - 1985

Fingerprint

Colony-Stimulating Factors
Lymphoma
Conditioned Culture Medium
DNA Replication
Granulocytes
Lectins
Bone Marrow Cells
Gel Chromatography
Molecular Weight
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Production of colony stimulating factor by EL-4 lymphoma cells stimulated with phytohemoagglutinin. / Pessina, Augusto; Brambilla, Paolo; Grazia Neri, Maria; Mocarelli, Paolo.

In: International Journal of Immunopharmacology, Vol. 7, No. 4, 1985, p. 549-553.

Research output: Contribution to journalArticle

Pessina, Augusto ; Brambilla, Paolo ; Grazia Neri, Maria ; Mocarelli, Paolo. / Production of colony stimulating factor by EL-4 lymphoma cells stimulated with phytohemoagglutinin. In: International Journal of Immunopharmacology. 1985 ; Vol. 7, No. 4. pp. 549-553.
@article{e0fbced2dcd348a2b1e2a44cfbd86e1f,
title = "Production of colony stimulating factor by EL-4 lymphoma cells stimulated with phytohemoagglutinin",
abstract = "Murine EL-4 lymphoma cells when stimulated with optimal doses of phytohemoagglutinin (PHA) release colony stimulating factor (CSF) in the conditioned medium. CSF production depends on PHA and stops when the lectin is withdrawn. The production is dependent on protein synthesis and is not related with DNA replication. The CSF produced by EL-4 cells stimulates murine bone marrow cells to differentiate in vitro into mature granulocytes. Unstimulated EL-4 cells do not produce CSF while its conditioned medium is able to inhibit EL-4 proliferation in vitro. Gel filtration chromatography of conditioned medium from PHA-stimulated EL4 cells yields a peak of activity corresponding to an apparent molecular weight of 20,000 - 25,000 daltons.",
author = "Augusto Pessina and Paolo Brambilla and {Grazia Neri}, Maria and Paolo Mocarelli",
year = "1985",
doi = "10.1016/0192-0561(85)90075-X",
language = "English",
volume = "7",
pages = "549--553",
journal = "International Journal of Immunopharmacology",
issn = "0192-0561",
publisher = "Elsevier BV",
number = "4",

}

TY - JOUR

T1 - Production of colony stimulating factor by EL-4 lymphoma cells stimulated with phytohemoagglutinin

AU - Pessina, Augusto

AU - Brambilla, Paolo

AU - Grazia Neri, Maria

AU - Mocarelli, Paolo

PY - 1985

Y1 - 1985

N2 - Murine EL-4 lymphoma cells when stimulated with optimal doses of phytohemoagglutinin (PHA) release colony stimulating factor (CSF) in the conditioned medium. CSF production depends on PHA and stops when the lectin is withdrawn. The production is dependent on protein synthesis and is not related with DNA replication. The CSF produced by EL-4 cells stimulates murine bone marrow cells to differentiate in vitro into mature granulocytes. Unstimulated EL-4 cells do not produce CSF while its conditioned medium is able to inhibit EL-4 proliferation in vitro. Gel filtration chromatography of conditioned medium from PHA-stimulated EL4 cells yields a peak of activity corresponding to an apparent molecular weight of 20,000 - 25,000 daltons.

AB - Murine EL-4 lymphoma cells when stimulated with optimal doses of phytohemoagglutinin (PHA) release colony stimulating factor (CSF) in the conditioned medium. CSF production depends on PHA and stops when the lectin is withdrawn. The production is dependent on protein synthesis and is not related with DNA replication. The CSF produced by EL-4 cells stimulates murine bone marrow cells to differentiate in vitro into mature granulocytes. Unstimulated EL-4 cells do not produce CSF while its conditioned medium is able to inhibit EL-4 proliferation in vitro. Gel filtration chromatography of conditioned medium from PHA-stimulated EL4 cells yields a peak of activity corresponding to an apparent molecular weight of 20,000 - 25,000 daltons.

UR - http://www.scopus.com/inward/record.url?scp=0021955744&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021955744&partnerID=8YFLogxK

U2 - 10.1016/0192-0561(85)90075-X

DO - 10.1016/0192-0561(85)90075-X

M3 - Article

C2 - 3876297

AN - SCOPUS:0021955744

VL - 7

SP - 549

EP - 553

JO - International Journal of Immunopharmacology

JF - International Journal of Immunopharmacology

SN - 0192-0561

IS - 4

ER -