Production of granulocyte-macrophage colony-stimulating factor but not IL- 3 by normal and neoplastic human B lymphocytes

S. Zupo, B. Perussia, L. Baldi, A. Corcione, M. Dono, M. Ferrarini, V. Pistoia

Research output: Contribution to journalArticle

Abstract

The ability of human B cells to produce granulocyte-macrophage (GM)-CSF and IL-3 was investigated. B cells, isolated from tonsils or from the peripheral blood of patients with B cell chronic lymphocytic leukemia using mAb and immune rosettes, were cultured with or without Staphylococcus aureus Cowan strain I. GM-CSF and IL-3 were measured in the culture supernatants using a bioassay based on the selective proliferative response of the MO7e megakaryoblastic cell line to IL-3 or GM-CSF. S. aureus Cowan I-stimulated normal B cells released measurable amounts of GM-CSF but not of IL-3 as determined in neutralization assays with specific mAb in the MO7e cell line test. Some of the unstimulated normal B suspensions also produced GM-CSF, albeit in lower quantities. When normal B cells were fractionated into small (resting) and large (activated) B cells by Percoll density gradients, spontaneous GM-CSF production was detected only in the large cell fractions, but small cells were induced to produce GM-CSF upon S. aureus Cowan I stimulation. On a per cell basis, tonsillar B cells were found capable of releasing more GM-CSF than activated peripheral blood monocytes. The amount of GM-CSF produced by B cells was always inferior to that released by stimulated peripheral blood T cells or NK cells. The purified B cell suspensions from all 14 B cell chronic lymphocytic leukemia patients studied released GM-CSF but not IL-3 in the culture supernatants, sometimes even in the absence of stimuli. Northern blot analysis on total or poly(A) + RNA confirmed the presence of GM-CSF, but not of IL-3, mRNA transcripts in both normal and malignant B cells. The results of these studies support the notion that activated human B lymphocytes release sufficient GM-CSF to play a role in the control of both hematopoiesis and the inflammatory process.

Original languageEnglish
Pages (from-to)1423-1430
Number of pages8
JournalJournal of Immunology
Volume148
Issue number5
Publication statusPublished - 1992

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Interleukin-3
Granulocyte-Macrophage Colony-Stimulating Factor
Granulocytes
B-Lymphocytes
Macrophages
Staphylococcus aureus
B-Cell Chronic Lymphocytic Leukemia
Suspensions
Cell Line
Messenger RNA
Palatine Tonsil
Hematopoiesis
Natural Killer Cells
Biological Assay
Northern Blotting
Monocytes
Blood Cells
Cell Count

ASJC Scopus subject areas

  • Immunology

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Production of granulocyte-macrophage colony-stimulating factor but not IL- 3 by normal and neoplastic human B lymphocytes. / Zupo, S.; Perussia, B.; Baldi, L.; Corcione, A.; Dono, M.; Ferrarini, M.; Pistoia, V.

In: Journal of Immunology, Vol. 148, No. 5, 1992, p. 1423-1430.

Research output: Contribution to journalArticle

Zupo, S. ; Perussia, B. ; Baldi, L. ; Corcione, A. ; Dono, M. ; Ferrarini, M. ; Pistoia, V. / Production of granulocyte-macrophage colony-stimulating factor but not IL- 3 by normal and neoplastic human B lymphocytes. In: Journal of Immunology. 1992 ; Vol. 148, No. 5. pp. 1423-1430.
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