Products of the lipoxygenase pathway in human natural killer cell cytotoxicity

Paolo Rossi, Jan Åke Lindgren, Charlotte Kullman, Mikael Jondal

Research output: Contribution to journalArticlepeer-review

Abstract

As earlier data suggested the importance of lipoxygenase activation for expression of human NK cell cytotoxicity, four different lipoxygenase inhibitors were tested for suppression of natural killer (NK) cell lysis. All inhibitors were found active at nontoxic concentrations with 50% inhibition at approximately 15 μM for nordihydroguaiaretic acid (NDGA). NK cell lysis could be reconstituted to NDGA-suppressed cells with leukotriene B4 (LTB4), the all-trans isomers 6-trans-LTB4 and 12-epi-6-trans-LTB4, and 20-COOH-LTB4. LTB4 reconstitution was best in the concentration range 1-100 pM and near control levels at both higher and lower concentrations. Herpesvirus Ateles-transformed killer T cells could also be inhibited by NDGA. These data indicate that lipoxygenase activity is required for human NK cell lysis and that several different LTB4-related products can restore NK activity in inhibited cells; they also suggest that the lipoxygenase pathway is present in the killer cell population.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalCellular Immunology
Volume93
Issue number1
DOIs
Publication statusPublished - 1985

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

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