Profiles of cancer stem cell subpopulations in cholangiocarcinomas

Vincenzo Cardinale, Anastasia Renzi, Guido Carpino, Alessia Torrice, Maria C. Bragazzi, Felice Giuliante, Agostino M. Derose, Alice Fraveto, Paolo Onori, Chiara Napoletano, Antonio Franchitto, Alfredo Cantafora, Gianluca Grazi, Nicola Caporaso, Giuseppe D'Argenio, Gianfranco Alpini, Lola M. Reid, Eugenio Gaudio, Domenico Alvaro

Research output: Contribution to journalArticle

Abstract

Cholangiocarcinomas (CCAs) comprise a mucin-secreting form, intrahepatic or perihilar, and a mixed form located peripherally. We characterized cancer stem cells (CSCs) in CCA subtypes and evaluated their cancerogenic potential. CSC markers were investigated in 25 human CCAs in primary cultures and established cell lines. Tumorigenic potential was evaluated in vitro or in xenografted mice after s.c. or intrahepatic injection in normal and cirrhotic (carbon tetrachloride-induced) mice. CSCs comprised more than 30% of the tumor mass. Although the CSC profile was similar between mucin-intrahepatic and mucin-perihilar subtypes, CD13+ CSCs characterized mixed-intrahepatic, whereas LGR5+ characterized mucin-CCA subtypes. Many neoplastic cells expressed epithelial-mesenchymal transition markers and coexpressed mesenchymal and epithelial markers. In primary cultures, epithelial-mesenchymal transition markers, mesenchymal markers (vimentin, CD90), and CD13 largely predominated over epithelial markers (CD133, EpCAM, and LGR5). In vitro, CSCs expressing epithelial markers formed a higher number of spheroids than CD13+ or CD90+ CSCs. In s.c. tumor xenografts, tumors dominated by stromal markers were formed primarily by CD90+ and CD13+ cells. By contrast, in intrahepatic xenografts in cirrhotic livers, tumors were dominated by epithelial traits reproducing the original human CCAs. In conclusion, CSCs were rich in human CCAs, implicating CCAs as stem cell-based diseases. CSC subpopulations generate different types of cancers depending on the microenvironment. Remarkably, CSCs reproduce the original human CCAs when injected into cirrhotic livers.

Original languageEnglish
Pages (from-to)1724-1739
Number of pages16
JournalAmerican Journal of Pathology
Volume185
Issue number6
DOIs
Publication statusPublished - Jun 1 2015

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Cholangiocarcinoma
Neoplastic Stem Cells
Mucins
Epithelial-Mesenchymal Transition
Neoplasms
Heterografts
Carbon Tetrachloride
Liver
Vimentin
Stem Cells
Cell Line
Injections

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cardinale, V., Renzi, A., Carpino, G., Torrice, A., Bragazzi, M. C., Giuliante, F., ... Alvaro, D. (2015). Profiles of cancer stem cell subpopulations in cholangiocarcinomas. American Journal of Pathology, 185(6), 1724-1739. https://doi.org/10.1016/j.ajpath.2015.02.010

Profiles of cancer stem cell subpopulations in cholangiocarcinomas. / Cardinale, Vincenzo; Renzi, Anastasia; Carpino, Guido; Torrice, Alessia; Bragazzi, Maria C.; Giuliante, Felice; Derose, Agostino M.; Fraveto, Alice; Onori, Paolo; Napoletano, Chiara; Franchitto, Antonio; Cantafora, Alfredo; Grazi, Gianluca; Caporaso, Nicola; D'Argenio, Giuseppe; Alpini, Gianfranco; Reid, Lola M.; Gaudio, Eugenio; Alvaro, Domenico.

In: American Journal of Pathology, Vol. 185, No. 6, 01.06.2015, p. 1724-1739.

Research output: Contribution to journalArticle

Cardinale, V, Renzi, A, Carpino, G, Torrice, A, Bragazzi, MC, Giuliante, F, Derose, AM, Fraveto, A, Onori, P, Napoletano, C, Franchitto, A, Cantafora, A, Grazi, G, Caporaso, N, D'Argenio, G, Alpini, G, Reid, LM, Gaudio, E & Alvaro, D 2015, 'Profiles of cancer stem cell subpopulations in cholangiocarcinomas', American Journal of Pathology, vol. 185, no. 6, pp. 1724-1739. https://doi.org/10.1016/j.ajpath.2015.02.010
Cardinale V, Renzi A, Carpino G, Torrice A, Bragazzi MC, Giuliante F et al. Profiles of cancer stem cell subpopulations in cholangiocarcinomas. American Journal of Pathology. 2015 Jun 1;185(6):1724-1739. https://doi.org/10.1016/j.ajpath.2015.02.010
Cardinale, Vincenzo ; Renzi, Anastasia ; Carpino, Guido ; Torrice, Alessia ; Bragazzi, Maria C. ; Giuliante, Felice ; Derose, Agostino M. ; Fraveto, Alice ; Onori, Paolo ; Napoletano, Chiara ; Franchitto, Antonio ; Cantafora, Alfredo ; Grazi, Gianluca ; Caporaso, Nicola ; D'Argenio, Giuseppe ; Alpini, Gianfranco ; Reid, Lola M. ; Gaudio, Eugenio ; Alvaro, Domenico. / Profiles of cancer stem cell subpopulations in cholangiocarcinomas. In: American Journal of Pathology. 2015 ; Vol. 185, No. 6. pp. 1724-1739.
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AU - Giuliante, Felice

AU - Derose, Agostino M.

AU - Fraveto, Alice

AU - Onori, Paolo

AU - Napoletano, Chiara

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AU - Cantafora, Alfredo

AU - Grazi, Gianluca

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