Profiling the proteome of exhaled breath condensate in healthy smokers and COPD patients by LC-MS/MS

Marco Fumagalli, Fabio Ferrari, Maurizio Luisetti, Jan Stolk, Pieter S. Hiemstra, Daniela Capuano, Simona Viglio, Laura Fregonese, Isa Cerveri, Federica Corana, Carmine Tinelli, Paolo Iadarola

Research output: Contribution to journalArticle

Abstract

Three pools of exhaled breath condensate (EBC) from non-smokers plus healthy smokers (NS + HS, n = 45); chronic obstructive pulmonary disease (COPD) without emphysema (COPD, n = 15) and subjects with pulmonary emphysema associated with α1-antitrypsin deficiency (AATD, n = 23) were used for an exploratory proteomic study aimed at generating fingerprints of these groups that can be used in future pathophysiological and perhaps even clinical research. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was the platform applied for this hypothesis-free investigation. Analysis of pooled specimens resulted in the production of a 'fingerprint' made of 44 proteins for NS/HS; 17 for COPD and 15 for the group of AATD subjects. Several inflammatory cytokines (IL-1α, IL-1β, IL-2; IL-12, α and β subunits, IL-15, interferon α and γ, tumor necrosis factor α); Type I and II cytokeratins; two SP-A isoforms; Calgranulin A and B and α1-antitrypsin were detected and validated through the use of surface enhanced laser-desorption ionization mass spectrometry (SELDI-MS) and/or by Western blot (WB) analysis. These results are the prelude of quantitative studies aimed at identifying which of these proteins hold promise as identifiers of differences that could distinguish healthy subjects from patients.

Original languageEnglish
Pages (from-to)13894-13910
Number of pages17
JournalInternational Journal of Molecular Sciences
Volume13
Issue number11
DOIs
Publication statusPublished - 2012

Keywords

  • COPD
  • EBC
  • LC-MS/MS
  • Proteomics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

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  • Cite this

    Fumagalli, M., Ferrari, F., Luisetti, M., Stolk, J., Hiemstra, P. S., Capuano, D., Viglio, S., Fregonese, L., Cerveri, I., Corana, F., Tinelli, C., & Iadarola, P. (2012). Profiling the proteome of exhaled breath condensate in healthy smokers and COPD patients by LC-MS/MS. International Journal of Molecular Sciences, 13(11), 13894-13910. https://doi.org/10.3390/ijms131113894