TY - JOUR
T1 - Progenitor cells from the adult mouse brain acquire a neuronal phenotype in response to β-amyloid
AU - Calafiore, M.
AU - Battaglia, G.
AU - Zappalà, A.
AU - Trovato-Salinaro, E.
AU - Caraci, F.
AU - Caruso, M.
AU - Vancheri, C.
AU - Sortino, M. A.
AU - Nicoletti, F.
AU - Copani, A.
PY - 2006/4
Y1 - 2006/4
N2 - Neurospheres from adult mouse subventricular zone (SVZ) were grown in suspension cultures for 12-15 days. Neurospheres consisted mainly of neural precursor cells (NPCs) immunoreactive for nestin and also contained nestin-negative precursors. We used these neurospheres to determine the effects of synthetic β-amyloid fragments (both βAP(1-42) and βAP(25-35)) on NPC proliferation, differentiation and survival. We show that neurospheres exposed to 25 μM βAP(25-35) or βAP(1-42) for 24 h (a toxic condition for mature neurons) did not undergo apoptosis. Instead, βAP(25-35) orientated nestin-negative precursors towards nestin-positive NPCs and turned nestin-positive NPCs into neuroblasts. Intracerebroventricular infusion of full-length βAP(1-42) increased the population of PSA-NCAM-positive cells in the SVZ, without affecting proliferation. We conclude that βAP influences the fate of progenitor cells, driving their differentiation towards a neuronal lineage.
AB - Neurospheres from adult mouse subventricular zone (SVZ) were grown in suspension cultures for 12-15 days. Neurospheres consisted mainly of neural precursor cells (NPCs) immunoreactive for nestin and also contained nestin-negative precursors. We used these neurospheres to determine the effects of synthetic β-amyloid fragments (both βAP(1-42) and βAP(25-35)) on NPC proliferation, differentiation and survival. We show that neurospheres exposed to 25 μM βAP(25-35) or βAP(1-42) for 24 h (a toxic condition for mature neurons) did not undergo apoptosis. Instead, βAP(25-35) orientated nestin-negative precursors towards nestin-positive NPCs and turned nestin-positive NPCs into neuroblasts. Intracerebroventricular infusion of full-length βAP(1-42) increased the population of PSA-NCAM-positive cells in the SVZ, without affecting proliferation. We conclude that βAP influences the fate of progenitor cells, driving their differentiation towards a neuronal lineage.
KW - β-amyloid
KW - Differentiation
KW - Neuroprogenitor cells
KW - Subventricular zone
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UR - http://www.scopus.com/inward/citedby.url?scp=33344460074&partnerID=8YFLogxK
U2 - 10.1016/j.neurobiolaging.2005.03.019
DO - 10.1016/j.neurobiolaging.2005.03.019
M3 - Article
C2 - 15964102
AN - SCOPUS:33344460074
VL - 27
SP - 606
EP - 613
JO - Neurobiology of Aging
JF - Neurobiology of Aging
SN - 0197-4580
IS - 4
ER -