Progenitor cells trapped in marrow filters can reduce GvHD and transplant mortality

Dany Vicente, M. Podestà, A. Pitto, S. Pozzi, S. Lucchetti, T. Lamparelli, E. Tedone, A. Ibatici, O. Figari, F. Frassoni, M. T. Van Lint, G. Piaggio, N. Sacchi, A. Bacigalupo

Research output: Contribution to journalArticlepeer-review

Abstract

A bone marrow harvest is filtered either in the operating room, in the laboratory or during infusion to the patient. Filters are usually discarded. Little is known of haemopoietic progenitor cells (HPCs) trapped in the filters. The aim of the study was to evaluate HPC content in the filters and to assess the outcome of transplants with filter-discarded or filter-recovered cells. Haemopoietic progenitors were grown from filters of 19 marrow transplants. We then compared the outcome of 39 filter-recovered transplants from HLA-identical siblings (years 2001-2004) with a matched cohort of 43 filter-discarded marrow grafts (years 1997-2000). Filters contained on average 21% long-term culture-initiating cells (LTC-IC) and 15% fibroblasts colony-forming units (CFU-F) of the total progenitor cell content. Filter-discarded transplants had significantly more grade II-IV graft-versus-host disease (GvHD) (42 vs 15%, P = 0.008) as compared to filter-recovered transplants, and more transplant-related mortality (TRM) (20 vs 3%, P = 0.04). The actuarial survival at 5 years is 69 vs 87%, respectively (P = 0.15). This study suggests that a significant proportion of LTC-IC is lost in the filters together with CFU-F. Recovery and add back of progenitors trapped in the filters may reduce GvHD and TRM.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalBone Marrow Transplantation
Volume38
Issue number2
DOIs
Publication statusPublished - Jul 2 2006

Keywords

  • Bone marrow filter
  • GvHD
  • Haemopoietic progenitor cells
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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