Progenitor/stem cell markers in brain adjacent to glioblastoma: GD3 ganglioside and NG2 proteoglycan expression

Gina Lama, Annunziato Mangiola, Gabriella Proietti, Anna Colabianchi, Cristiana Angelucci, Alessio D'Alessio, Pasquale De Bonis, Maria Concetta Geloso, Libero Lauriola, Elena Binda, Filippo Biamonte, Maria Grazia Giuffrida, Angelo Vescovi, Gigliola Sica

Research output: Contribution to journalArticle

Abstract

Characterization of tissue surrounding glioblastoma (GBM) is a focus for translational research because tumor recurrence invariably occurs in this area. We investigated the expression of the progenitor/ stem cell markers GD3 ganglioside and NG2 proteoglycan in GBM, peritumor tissue (brain adjacent to tumor, BAT) and cancer stem-like cells (CSCs) isolated from GBM (GCSCs) and BAT (PCSCs). GD3 and NG2 immunohistochemistry was performed in paired GBM and BAT specimens from 40 patients. Double-immunofluorescence was carried out to characterize NG2-positive cells of vessel walls. GD3 and NG2 expression was investigated in GCSCs and PCSCs whose tumorigenicity was also evaluated in Scid/bg mice. GD3 and NG2 expression was higher in tumor tissue than in BAT. NG2 decreased as the distance from tumor margin increased, regardless of the tumor cell presence, whereas GD3 correlated with neoplastic infiltration. In BAT, NG2 was coexpressed with a-smooth muscle actin (α-SMA) in pericytes and with nestin in the endothelium. Higher levels of NG2 mRNA and protein were found in GCSCs while GD3 synthase was expressed at similar levels in the 2 CSC populations. PCSCs had lower tumorigenicity than GCSCs. These data suggest the possible involvement of GD3 and NG2 in pre/pro-tumorigenic events occurring in the complex microenvironment of the tissue surrounding GBM.

Original languageEnglish
Pages (from-to)134-147
Number of pages14
JournalJournal of Neuropathology and Experimental Neurology
Volume75
Issue number2
DOIs
Publication statusPublished - 2016

Keywords

  • Brain adjacent to tumor
  • Cancer stem cells
  • GD3 ganglioside
  • Glioblastoma
  • NG2 proteoglycan

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Clinical Neurology
  • Neurology
  • Cellular and Molecular Neuroscience

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  • Cite this

    Lama, G., Mangiola, A., Proietti, G., Colabianchi, A., Angelucci, C., D'Alessio, A., De Bonis, P., Geloso, M. C., Lauriola, L., Binda, E., Biamonte, F., Giuffrida, M. G., Vescovi, A., & Sica, G. (2016). Progenitor/stem cell markers in brain adjacent to glioblastoma: GD3 ganglioside and NG2 proteoglycan expression. Journal of Neuropathology and Experimental Neurology, 75(2), 134-147. https://doi.org/10.1093/jnen/nlv012