Progesterone induced expression of alkaline phosphatase is associated with a secretory phenotype in T47D breast cancer cells

D. Di Lorenzo, M. Gianni, G. F. Savoldi, F. Ferrari, A. Albertini, E. Garattini

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In our previous work we reported on stimulation of a tissue unspecific alkaline phosphatase (liver/bone/kidney, L/B/K) in the human breast cancer cell line T47D by progestins. Here we show that in these cells the synthetic progestin R5020 (Promegestone) induces transcription of a 2.7-kilobase ALP mRNA. In this cell line, maximal induction is reached after 24 hours, decreases to 50 % after 72 hours and is sensitive to inhibitors of protein synthesis. The induction of ALP mRNA and enzyme activity is specific for R5020 and dexamethasone and is completely inhibited in the presence of 10 - 7 M RU486. When treated with R5020 for 48 hours, T47D cells exhibit a substantially altered phenotype, lipid vacuoles accumulate all over the cytoplasm, conferring to the cells a secretory morphology. This phenotype is associated with increased ALP enzyme activity which is also maximal at 48 hours. This effect is progestin specific since other steroids do not lead to the same macroscopical changes.

Original languageEnglish
Pages (from-to)1066-1072
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume192
Issue number3
DOIs
Publication statusPublished - 1993

Fingerprint

Promegestone
Enzyme activity
Progestins
Progesterone
Alkaline Phosphatase
Cells
Progesterone Congeners
Breast Neoplasms
Phenotype
Messenger RNA
Protein Synthesis Inhibitors
Transcription
Liver
Dexamethasone
Bone
Steroids
Tissue
Lipids
Cell Line
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Progesterone induced expression of alkaline phosphatase is associated with a secretory phenotype in T47D breast cancer cells. / Di Lorenzo, D.; Gianni, M.; Savoldi, G. F.; Ferrari, F.; Albertini, A.; Garattini, E.

In: Biochemical and Biophysical Research Communications, Vol. 192, No. 3, 1993, p. 1066-1072.

Research output: Contribution to journalArticle

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AU - Garattini, E.

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