Prognostic relevance of C-myc gene expression in giant-cell tumor of bone

G. Gamberi, M. S. Benassi, T. Bohling, P. Ragazzini, L. Molendini, M. R. Sollazzo, M. Merli, C. Ferrari, G. Magagnoli, F. Bertoni, P. Picci

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Abstract

Giant-cell tumor is a primary bone tumor, of uncertain origin, with the potential capacity to metastasize. To study the role of c-myc and c-fos oncogene overexpression in the tumorigenesis and metastatic spread of giant- cell tumors, 32 primary tumors were collected; of these, 19 remained disease-free and 13 metastasized to the lung. Samples of lung metastasis from these 13 patients were also available for study. The expression of c- myc and c-fos mRNA was studied by reverse transcription-polymerase chain reaction and by in situ hybridization. The expression of protein was studied by Western blot analysis and by immunohistochemistry. C-myc mRNA was overexpressed in 12 (38%) of the 32 primary tumors. Thirteen primary tumors metastasized to the lung; in nine (69%) of these, c-myc mRNA was overexpressed. The c-myc protein was overexpressed in seven (54%) of the 13 tumors that metastasized to the lung. C-fos was overexpressed in only one lung metastasis. A strong correlation between the overexpression of c-myc and the occurrence of metastases was found; thus, c-myc seems a powerful prognosticator in giant-cell tumor. C-myc was overexpressed both in giant cells and in mononuclear cells, suggesting that both cell types are involved in the progression of this tumor.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Orthopaedic Research
Volume16
Issue number1
DOIs
Publication statusPublished - Jan 1998

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ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

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