Prognostic role of cyclin d1 in lung cancer relationship to proliferating cell nuclear antigen

Mario Caputi, Angela M. Groeger, Vincenzo Esposito, Charity Dean, Antonio De Luca, Carmen Pacilio, Michael R. Muller, Giovan G. Giordano, Feliciano Baldi, Ernst Wolner, Antonio Giordano

Research output: Contribution to journalArticle

Abstract

We developed an immunohistochemical assay specific for cyclin D1 and suitable for formalin-fixed and paraffin-embedded sections, to evaluate cyclin D1 expression in a group of 135 surgically resected lung-cancer patients for the purpose of investigating the prognostic role of this protein in lung cancer. In addition, we compared cyclin D1 expression with the expression of proliferating cell nuclear antigen (PCNA), considered to be a reliable index of the proliferation rate. We found cyclin D1 expressed in more than 60% of the neoplastic cells in 26.5% of our specimens. A total of 24.5% of the specimens showed cyclin D1 expression in a percentage of cells ranging from 30 to 60%; 36.7% of the specimens expressed cyclin D1 in less than 30% of the cells; and 12.2% of the specimens expressed cyclin D1 in less than 1% of the evaluated cells. Western blot analyses confirmed the specificity of this assay by correlating statistically in a highly significant fashion with the immunohistochemical results (P = 0.0003). Furthermore, we found a direct relationship between cyclin D1 and PCNA immunodetection (P = 0.0004), which correlated cyclin D1 overexpression with a higher tumor proliferation rate. When we analyzed our data statistically, cyclin D1 expression was found to be a negative prognostic marker (P <0.00005) whose expression correlates with a shorter patient survival time.

Original languageEnglish
Pages (from-to)746-750
Number of pages5
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume20
Issue number4
Publication statusPublished - 1999

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ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Caputi, M., Groeger, A. M., Esposito, V., Dean, C., De Luca, A., Pacilio, C., Muller, M. R., Giordano, G. G., Baldi, F., Wolner, E., & Giordano, A. (1999). Prognostic role of cyclin d1 in lung cancer relationship to proliferating cell nuclear antigen. American Journal of Respiratory Cell and Molecular Biology, 20(4), 746-750.