Prognostic significance of fluorescent In Situ hybridisation in the follow-up of non-muscle-invasive bladder cancer

Massimo Maffezzini, Fabio Campodonico, Giacomo Capponi, Giorgio Canepa, Stefania Casazza, Roberto Bandelloni, Stefania Tamagno, Matteo Puntoni

Research output: Contribution to journalArticlepeer-review


Aim: To evaluate the potential contribution of a fluorescent in situ hybridization (FISH) as prognostic indicator of the risk of recurrence or progression in patients undergoing follow-up for non-muscle-invasive bladder cancer (NMIBC). Patients and Methods: A total of 126 consecutive patients with a history of NMIBC being followed-up with urinary cytology and cystoscopy at a referral centre were studied. Patients with carcinoma in situ, or tumour stage higher than pT1 were excluded. A UroVysion FISH kit was used to detect four chromosomal abnormalities, specifically, locus 9p21, Ch 3, 7, and 17. Three FISH patterns were defined: negative; low-risk positive, i.e. positive staining for 9p21 and/or Ch3 abnormalities; and high-risk positive, i.e. positive staining for Ch7 andlor 17. Results: Overall 73 out of 126 patients (57.9%) had a positive urinary FISH test. After a median time of 14 months, 46 FISH-positive patients underwent recurrence (36.5%) and in 15 patients there was progression of disease (11.9%). Among positive patients, the low-risk category was found in 34, and the high-risk in 39. Low-risk FISH-positive patients had a higher rate of recurrence as compared to FISH-negative patients, with a hazard ratio (HR) of 1.6. The recurrence rate was even greater in patients with a high-risk positive test, with an HR of 1.9. The limitation of the study was that the impact of intravesical treatment was not assessed. Conclusion: The urinary FISH test can be used as an aid in predicting the risk of recurrence during follow-up of patients with history of NMIBC.

Original languageEnglish
Pages (from-to)4761-4765
Number of pages5
JournalAnticancer Research
Issue number11
Publication statusPublished - Nov 2010


  • Bladder caner
  • Prognosis
  • Recurrence
  • Urinary fish test
  • UroVysion assay

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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