Prognostic value of beta-tubulin-3 and c-Myc in muscle invasive urothelial carcinoma of the bladder

Francesco Massari, Emilio Bria, Chiara Ciccarese, Enrico Munari, Alessandra Modena, Valentina Zambonin, Isabella Sperduti, Walter Artibani, Liang Cheng, Guido Martignoni, Giampaolo Tortora, Matteo Brunelli

Research output: Contribution to journalArticle

Abstract

Background: To date, putative prognostic biomarkers have shown limited utility from the clinical perspective for bladder urothelial carcinoma. Herein, the expression of beta-tubulin-3 and c-Myc was evaluated to determine their prognostic potential. Methods: In formalin fixed-paraffin embedded blocks, immunohistochemical expression of c-Myc and beta-tubulin-3 was evaluated. H score ranging from 0 to 300 was obtained by multiplying the percentage of positive cells by intensity (0-3); c-Myc and beta-tubulin-3 expression was defined: 0: negative, 1: weakly positive, 2: strongly positive. Results: beta-tubulin-3 and c-Myc immunoexpression was available for 46 cases. At the univariate analysis, node-involvement, beta-tubulin-3 and c-Myc overexpression discriminate shorter DFS (HR 2.19, p = 0.043; HR 3.10, p = 0.24 and HR 3.05, p = 0.011, respectively); 2-yrs DFS log-rank analysis according to low versus high level of immunoexpression were statistically significant; beta-tubulin-3, 53% low vs 12.7% high (p = value 0.02) and c-Myc 28 low vs 8 high (p-value 0.007). Patients displaying negative beta-tubulin-3/c-Myc had statistically significant better 2-yrs DFS than those with mixed expression or double positivity (54.5% versus 18.7% versus 0%, log-rank p = 0.006). Conclusions: c-Myc and beta-tubulin-3 show improvement for prognostic risk stratification in patients with muscle invasive bladder urothelial carcinoma. These molecular pathways may also be candidate to improve predictiveness to targeted therapies.

Original languageEnglish
Article numbere0127908
JournalPLoS One
Volume10
Issue number6
DOIs
Publication statusPublished - Jun 5 2015

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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    Massari, F., Bria, E., Ciccarese, C., Munari, E., Modena, A., Zambonin, V., Sperduti, I., Artibani, W., Cheng, L., Martignoni, G., Tortora, G., & Brunelli, M. (2015). Prognostic value of beta-tubulin-3 and c-Myc in muscle invasive urothelial carcinoma of the bladder. PLoS One, 10(6), [e0127908]. https://doi.org/10.1371/journal.pone.0127908