Programmed cell death 4 (PDCD4) expression during multistep Barrett's carcinogenesis

Matteo Fassan, Marco Pizzi, Giorgio Battaglia, Luciano Giacomelli, Paola Parente, Paolo Bocus, Ermanno Ancona, Massimo Rugge

Research output: Contribution to journalArticle

Abstract

Aim: To test the contribution of programmed cell death 4 (PDCD4) tumour suppressor gene in Barrett's carcinogenesis. Methods: PDCD4 immunohistochemical expression was assessed in 88 biopsy samples obtained from histologically proven long-segment Barrett's mucosa (BM; 25 non-intestinal columnar metaplasia, 25 intestinal metaplasia (IM), 16 low-grade intraepithelial neoplasia (LG-IEN), 12 high-grade IEN (HG-IEN) and 10 Barrett's adenocarcinoma (BAc)). As controls, 25 additional samples of native oesophageal mucosa (N) were obtained from patients with dyspepsia. To further support the data, the expression levels of miR-21, an important PDCD4 expression regulator, in 14 N, 5 HG-IEN and 11 BAc samples were determined by quantitative real-time PCR analysis. Results: PDCD4 immunostaining decreased progressively and significantly with the progression of the phenotypic changes occurring during Barrett's carcinogenesis (p

Original languageEnglish
Pages (from-to)692-696
Number of pages5
JournalJournal of Clinical Pathology
Volume63
Issue number8
DOIs
Publication statusPublished - Aug 2010

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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