Programming of Intestinal Epithelial Differentiation by IL-33 Derived from Pericryptal Fibroblasts in Response to Systemic Infection

Mousumi Mahapatro, Sebastian Foersch, Manuela Hefele, Gui Wei He, Elisa Giner-Ventura, Tamar Mchedlidze, Markus Kindermann, Stefania Vetrano, Silvio Danese, Claudia Günther, Markus F. Neurath, Stefan Wirtz, Christoph Becker

Research output: Contribution to journalArticlepeer-review

Abstract

The intestinal epithelium constitutes an efficient barrier against the microbial flora. Here, we demonstrate an unexpected function of IL-33 as a regulator of epithelial barrier functions. Mice lacking IL-33 showed decreased Paneth cell numbers and lethal systemic infection in response to Salmonella typhimurium. IL-33 was produced upon microbial challenge by a distinct population of pericryptal fibroblasts neighboring the intestinal stem cell niche. IL-33 programmed the differentiation of epithelial progenitors toward secretory IEC including Paneth and goblet cells. Finally, IL-33 suppressed Notch signaling in epithelial cells and induced expression of transcription factors governing differentiation into secretory IEC. In summary, we demonstrate that gut pericryptal fibroblasts release IL-33 to translate bacterial infection into an epithelial response to promote antimicrobial defense.

Original languageEnglish
Pages (from-to)1743-1756
Number of pages14
JournalCell Reports
Volume15
Issue number8
DOIs
Publication statusPublished - May 24 2016

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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