Progress in the ex vivo expansion of hematopoietic progenitors

Marcus O. Muench, Maria Grazia Roncarolo, Reiko Namikawa, Alicia Bárcenat, Malcolm A S Moore

Research output: Contribution to journalArticle

Abstract

In this review we describe how studies on the cytokine-stimulated growth of murine bone marrow (BM) progenitors have lead to the observations that large increases in progenitor numbers can be achieved in short-term cytokine-stimulated liquid cultures. Transplantation of these ex vivo expanded murine BM cells was shown to decrease the number of BM cells required to confer radioprotection and to increase the recovery rate of both myeloid and erythroid peripheral blood cells. The ex vivo expansion of murine BM cells does not, however, markedly diminish stem cells capable of long-term hematopoietic reconstitution. Investigations on the expansion of human BM, peripheral blood, umbilical cord blood and fetal hematopoietic progenitors have demonstrated that clinically useful increases in progenitor numbers from these tissues are possible. Thus, ex vivo progenitor expansion may soon be of use in transplantation protocols to accelerate hematopoietic reconstitution and in gene therapy protocols if hematopoietic stem cells can be maintained during ex vivo culture.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalLeukemia and Lymphoma
Volume16
Issue number1-2
DOIs
Publication statusPublished - 1994

Keywords

  • Bone marrow expansion
  • Bone marrow transplantation
  • Cytokines cord blood
  • Fetal liver
  • Hematopoiesis

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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    Muench, M. O., Roncarolo, M. G., Namikawa, R., Bárcenat, A., & Moore, M. A. S. (1994). Progress in the ex vivo expansion of hematopoietic progenitors. Leukemia and Lymphoma, 16(1-2), 1-11. https://doi.org/10.3109/10428199409114134